Hello Hamidreza. There are different ways to approach this. First of all, as for any EAE experiment you will need to follow body weight changes of your mice daily. Your animal license will specify the limit of body weight loss that is acceptable before you need to terminate your animals due to a humane endpoint. But you may want to provide fluid supplementation via i.p. injection of sterile saline (typically 1 ml) for mice reaching significant weight loss (e.g. >10% initial body weight at immunization), but which did not reach the humane endpoint, which can be a strong indication that they may be dehydrated. You can also implement this action when you notice that due to their level of neuromotor disability they will have difficulty drinking water, regardless of a body weight loss. You may also consider placing some food pellets inside the cage if you anticipate that in any particular case there might be a mouse having difficulty reaching out for food, because access to water may not be the single issue for them. One alternative is to include special type of food in gel format which you can buy from specialized vendors (see https://www.clearh2o.com/product/dietgel-recovery/), which would allow for mice to replenish for water and food at the same time, even in case of hindlimb paralysis. Whatever your choice, remember that all this needs to be discussed with your local ethics committee before you implement it in your studies. You need to be specially careful with the mice during the acute phase of EAE, looking after them at least twice a day.