I kept Interleukin 17 kit at freezing temperature for 24 hours instead of being stored at 4-8 ° C. Can I use this kit or is the kit corrupted?

More Hamidreza Safari's questions See All

I want to know that the start of the definition begins with the abstract or the introduction?

To write an article, abbreviations should be defined After that, it can be used only the abbreviation. I want to know that the start of the definition begins with the abstract or the introduction?

31 December 2018 840 3 View

I use Endnote softwar. Is there any Website or software to provide journals citation style?

The citation styles of Some journals are available, but others are not. Is there a complete source for providing journals citation styles ??? I try to obtain the citation style of Nutritional...

09 October 2018 6,860 5 View

Can I use human FTY720 drug to treat EAE model in C57 mice?

Is it FTY720 specific to humans or it can be used to treat EAE in mice as a control positive ?

02 March 2018 4,552 3 View

How is the Stimulation index calculation?

I want to evaluate the effect of a medication on a EAE mouse model and I defined the control positive,control negative and treatment group. If i want to separate Spleen mononuclear cells and...

02 March 2018 736 2 View

What is the cause of weight loss in the C57 EAE model???

I would like to know the cause weight loss of mice in theC57 EAE model.

02 March 2018 2,315 0 View

What is difference between therapeutic and prophylactic group in research ?

I want to evaluate the therapeutic and prophylactic of a drug on RA model in rat. when i should started to administration of drug in therapeutic and prophylactic group? in some studies they...

02 March 2018 4,767 5 View

Which spleen mononuclear cells in culture plate attached to the bottom plate?

In some studies to measure cytokines in the supernatant for the culture of spleen mononuclear cells, after a while, the suspensions cells are separated from sticky cells and cultivated on a...

01 February 2018 3,494 3 View

I need to find a collaborator corresponding author to write a review.How can a good corresponding author be found?

I want to write a review article about inflammation or MS, but I need a good corresponding author. How can a good corresponding author find??

11 December 2017 909 3 View

Is it possible the contamination of cells in nitrogen tank( -196C)?

I want to know if I storage the cells that were not sterile, in nitrogen tank, is it possible the growth of very low of contamination in this temperature ?

11 December 2017 7,256 1 View

I induce EAE in C57 mice and it is hard for them drinking water.How can i solve this problem?

How can i solve drinking water problem in EAE mice model?

11 December 2017 4,678 1 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

Melt curve problem in qPCR?

Do these peaks in the curve indicate primer dimer or non-specific products?

01 August 2024 7,466 0 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Compress Information Neurally?

Samuel Morse, the inventor of the Morse Code, understood that certain letters in the English language occurred more frequently than others (Gallistel and King 2010). To deal with this, Morse used...

01 August 2024 4,456 2 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Can we use technical replicates for statistical analysis of multiplex cytokine data?

I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....

31 July 2024 3,665 3 View

What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

How can we improve storage conditions of museum objects?

90% of museum objects worldwide are in storage.

21 July 2024 8,038 6 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

Muhannad Alruwaili

I Icannot tell you for sure, but you can try the kit using standard with known conc.

Ali Abdulhussain Mahdi

yes , can used it and use standers to removed any errors

Weam Al-Hamadany

it might be affected, but you can melt its standard gradually to avoid heat shock effects..furthermore; you can use one blank well to correct errors.

Hiba Riyadh Al-abodi

following