The EASTBIOPHARM company recommended that kit storage at 2-8C. This mouse cytokine kit frozen for 24 hours. I want to know can i use it?
I Icannot tell you for sure, but you can try the kit using standard with known conc.
yes , can used it and use standers to removed any errors
it might be affected, but you can melt its standard gradually to avoid heat shock effects..furthermore; you can use one blank well to correct errors.
following
To write an article, abbreviations should be defined After that, it can be used only the abbreviation. I want to know that the start of the definition begins with the abstract or the introduction?
31 December 2018 717 3 View
The citation styles of Some journals are available, but others are not. Is there a complete source for providing journals citation styles ??? I try to obtain the citation style of Nutritional...
09 October 2018 6,724 5 View
Is it FTY720 specific to humans or it can be used to treat EAE in mice as a control positive ?
02 March 2018 4,413 3 View
I want to evaluate the effect of a medication on a EAE mouse model and I defined the control positive,control negative and treatment group. If i want to separate Spleen mononuclear cells and...
02 March 2018 555 2 View
I would like to know the cause weight loss of mice in theC57 EAE model.
02 March 2018 2,185 0 View
I want to evaluate the therapeutic and prophylactic of a drug on RA model in rat. when i should started to administration of drug in therapeutic and prophylactic group? in some studies they...
02 March 2018 4,594 5 View
In some studies to measure cytokines in the supernatant for the culture of spleen mononuclear cells, after a while, the suspensions cells are separated from sticky cells and cultivated on a...
01 February 2018 3,360 3 View
I want to write a review article about inflammation or MS, but I need a good corresponding author. How can a good corresponding author find??
11 December 2017 774 3 View
I want to know if I storage the cells that were not sterile, in nitrogen tank, is it possible the growth of very low of contamination in this temperature ?
11 December 2017 7,126 1 View
How can i solve drinking water problem in EAE mice model?
11 December 2017 4,498 1 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Anyone can recommend any books on such topics? Thanks in advance.
02 March 2021 8,457 2 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.
28 February 2021 6,512 3 View
I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.
28 February 2021 5,929 2 View
We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...
28 February 2021 1,149 1 View
The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...
27 February 2021 6,350 2 View
Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...
25 February 2021 2,442 1 View