A TEER electrode is typically used to test the integrity of endothelial cell layers. Does anyone know how to design your own TEER electrode using things like a volt meter?
Thanks,
Gloria
I conduct unit single recording, the pipettes are very different
sorry, maybe my colleague know
Usually TEER is measured with the 4 electrode configuration, as in the EVOM chopsticks, where you need to inject a current and measure voltage. TEER is difficult to measure precisely unless your electrodes are always in the same position relative to your cell layer. So unless you are an expert in electrical engineering, I suggest you use commercial electrodes. I can indicate a couple of papers and theses to you if you would like to try.
You don’t need to be an expert in electrical engineering. We have recently published a video protocol to show how a cost-effective (approx. 150EUR) and customizable TEER measurement device can be self-assembled.
Theile M, et al. A simple approach to perform TEER measurement using a self-made volt-amperemeter with programmable output frequency. J. Vis. Exp., 2019, https://www.jove.com/video/60087
Here is our paper on TEER and TEEI (transepithelial electrical impedance): Cacopardo et al, Real-time cellular impedance monitoring and imaging of biological barriers in a dual-flow membrane bioreactor. Biosensors and Bioelectronics,Volume 140, 1 September 2019, 111340.
Article Real-time cellular impedance monitoring and imaging of biolo...