Have any one tried to extract RNA from 72 hour old sample
Not really. In my case it was 68 years old sample.
Dear
Not experience concerning this case.
hiiii,
How long do they take before initial disicion ?
04 May 2019 5,762 2 View
what is the minmum count to perform paired sample gene expression assay if my cases have pre and post reading eg I have 4 cases with low expression and 6 cases of high expression baseline and...
11 December 2018 5,711 1 View
hi all , we have Naviuos FC from Beckan Coulter. I had got alarm saying ( sample pressure error) but there was normal aquisition , have any one expirence the same error ?
03 April 2018 8,735 0 View
I use free software from image j to analyse microscopic pictures , but , I cant use the IHC tool properly as well as I cant undestand some features. do any one have tutorial videos to use the program?
03 April 2018 3,393 4 View
I wanr to compare differnt categerical varibles on gene expression ( hig , low) can I use linear rigression ?
02 March 2018 6,597 3 View
How to compare un normalized RT PCR DATA Between cases and control (relative quatitation)
01 February 2018 8,213 3 View
hellow all When to use Delta CT vs DD CT in gene expression assay
31 December 2017 9,412 3 View
Hi all I use total RNA EXTRACTION KIT ( QIAGEN). THEN I USE SYBR GREEN BASE RT PCR to antiquate HOTAIR LncRNA What is the accepted change in Tm between run of my product
11 December 2017 2,746 1 View
I use 1ug of total RNA in cDNA reaction using revert aid first starnd cDNA synthesis then I use 1ul in qpcr reaction (10 ul volume) My HK gene usually less than 10 I measured thre cDNA using...
10 November 2017 7,558 4 View
Hi all I use a sybr green based assay . i sawproduct at ct 34 in NTC any recommendation ??
10 November 2017 1,919 8 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
Is this circle one of the rings?
03 August 2024 5,988 0 View
