Hi all has any one tried PCR data analysis through free online thermofisher cloud
Regarding data privacy
How long do they take before initial disicion ?
04 May 2019 5,635 2 View
what is the minmum count to perform paired sample gene expression assay if my cases have pre and post reading eg I have 4 cases with low expression and 6 cases of high expression baseline and...
11 December 2018 5,594 1 View
hi all , we have Naviuos FC from Beckan Coulter. I had got alarm saying ( sample pressure error) but there was normal aquisition , have any one expirence the same error ?
03 April 2018 8,621 0 View
I use free software from image j to analyse microscopic pictures , but , I cant use the IHC tool properly as well as I cant undestand some features. do any one have tutorial videos to use the program?
03 April 2018 3,275 4 View
I wanr to compare differnt categerical varibles on gene expression ( hig , low) can I use linear rigression ?
02 March 2018 6,478 3 View
How to compare un normalized RT PCR DATA Between cases and control (relative quatitation)
01 February 2018 8,016 3 View
hellow all When to use Delta CT vs DD CT in gene expression assay
31 December 2017 9,033 3 View
Hi all I use total RNA EXTRACTION KIT ( QIAGEN). THEN I USE SYBR GREEN BASE RT PCR to antiquate HOTAIR LncRNA What is the accepted change in Tm between run of my product
11 December 2017 2,644 1 View
I use 1ug of total RNA in cDNA reaction using revert aid first starnd cDNA synthesis then I use 1ul in qpcr reaction (10 ul volume) My HK gene usually less than 10 I measured thre cDNA using...
10 November 2017 7,413 4 View
Hi all I use a sybr green based assay . i sawproduct at ct 34 in NTC any recommendation ??
10 November 2017 1,768 8 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...
03 March 2021 961 3 View
I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...
03 March 2021 8,920 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...
03 March 2021 2,870 3 View
I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...
02 March 2021 4,362 1 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View