No flurecence at one PCR well?

More Fatma Abdel Wahab's questions See All

Anyone had experience in publication in Journal of applied genetics?

How long do they take before initial disicion ?

04 May 2019 5,635 2 View

Paired samples statistics?

what is the minmum count to perform paired sample gene expression assay if my cases have pre and post reading eg I have 4 cases with low expression and 6 cases of high expression baseline and...

11 December 2018 5,594 1 View

Flow cytometry alarming messages?

hi all , we have Naviuos FC from Beckan Coulter. I had got alarm saying ( sample pressure error) but there was normal aquisition , have any one expirence the same error ?

03 April 2018 8,621 0 View

Image analysis by image J?

I use free software from image j to analyse microscopic pictures , but , I cant use the IHC tool properly as well as I cant undestand some features. do any one have tutorial videos to use the program?

03 April 2018 3,275 4 View

Statistical method of data represntaton?

I wanr to compare differnt categerical varibles on gene expression ( hig , low) can I use linear rigression ?

02 March 2018 6,478 3 View

Un normalised q pcr ?

How to compare un normalized RT PCR DATA Between cases and control (relative quatitation)

01 February 2018 8,016 3 View

Delta CT in qPCR Reactions?

hellow all When to use Delta CT vs DD CT in gene expression assay

31 December 2017 9,033 3 View

LncRNA METING TEMPRATURE?

Hi all I use total RNA EXTRACTION KIT ( QIAGEN). THEN I USE SYBR GREEN BASE RT PCR to antiquate HOTAIR LncRNA What is the accepted change in Tm between run of my product

11 December 2017 2,644 1 View

What the optimum concentration of cDNA in rt pcr?

I use 1ug of total RNA in cDNA reaction using revert aid first starnd cDNA synthesis then I use 1ul in qpcr reaction (10 ul volume) My HK gene usually less than 10 I measured thre cDNA using...

10 November 2017 7,413 4 View

PCR amplified product?

Hi all I use a sybr green based assay . i sawproduct at ct 34 in NTC any recommendation ??

10 November 2017 1,768 8 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Ahmed A. Suleiman

May be the DNA destroyed in this period or your primers contam you can check the DNA by use another primer

Fatma Abdel Wahab

Dear Ahmed The Sample in the same run had also , other 4 genes screening

They all give Amplfication plot

so you can screen about mutation in the primer annealing region

Can it be two samples ?

Does this finding go with Sample evaopration?

Paul Rutland

As Ahmed says a polymorphism under either primer will result in no amplification or less likely a deletion of the amplimer or primer binding site ( homozygous if on an autosome) will also result in no amplification