hello
Iam doing an Mutation screening experiments byHRM .
the mutant variants should be then sequenced
Should I preserve the amplicon at -20 or I should run another reaction immediately before amplification
The PCR sample can be stored at -20. No problem there.
Both of your query are not relevant to each other.
All amplified RTPCR product can be stored at -20C and they can be sequenced at any time in future as per your convenience.
Dear Sanjay , I am asking as already in my reaction o ve used a fluorescent dye , should affect the sequencingv or not
thanks
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