How to prepare 75 uM nitro blue tetrazolium HCl prior to superoxide dismutase (SOD) assay?

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How to prepare OH- terminated Si Wafer?

Hi, Folks. I am working on the ALD project to deposit Al2O3 on the Si wafer from TMA and Water. I have struggled with the preparation of OH- terminated Si wafer. I have both Si(1 0 0) and...

08 February 2021 8,792 2 View

BrdU staining issue?

Hi everyone. I bumped into the difficulty that I couldn't get any signals from my brain section in the DG region of hippocampus. However, I have standardized the BrdU protocol in my brain...

13 January 2021 9,449 0 View

Real Time PCR: What is the justification for acceptable variance within 3 Ct? Is there any publication on this variance?

Hi all, I was not able to find any source with evidential justification for why the acceptable variance for internal control should be within 3 Ct and not other numbers of cycle of amplification....

17 December 2020 9,481 1 View

Methods for multivariate discrete-time time series clustering?

Can somebody suggest a popular method for clustering multivariate discrete-time time-series data ? My dataset is about water quality parameters taken in 6 stations by seasons in 5 years . Thank...

09 December 2020 6,933 12 View

Effective number of species and Wilcoxon Signed Rank Test for diversity between 2 ecosystems: is this accurate?

Hi! So I'm currently studying the diversity difference between 2 ecosystems. I ran a Shannon-Wiener test on site 1 and site 2 and found the indices of 1.525 and 2.165 , respectively. I have done...

22 November 2020 6,798 1 View

How to reproduce the sub-grade of pavements without using soil?

Dear colleagues, In order to evaluate the performance of concrete slabs-on-grade, the sub-grade should also be considered in the testing setup. Using soil or sand to reproduce the sub-grade is a...

20 November 2020 7,921 1 View

How to determination of available silicon in fertilizer by UV-Vis?

Hello everyone! I have a problem with when the determination of available silicon in fertilizer by UV-Vis. I do that following method A 5-Day Method for Determination of Soluble Silicon...

10 November 2020 1,370 10 View

What is the problem with my 10um Nissl stained cell?

Please see the attachment. In my case, I tried a lot of time on the Nissl staining on 10um cryosection. I am not sure which step goes wrong? I make sure my anti-rolling glass is intact. seem like...

07 November 2020 9,568 3 View

Where and how do I specify frequency in the route section of frequency-dependent calculation (Gaussian using Linux)?

Hello everyone. I would like to compute the dynamic hyperpolarizability value of a molecule using Gaussian on Linux OS. I have read that the essential keywords to include for such request are the...

07 November 2020 5,620 4 View

What type of regression should I be using?

I am planning to conduct a study with the following variables: A - independent variable B - intervening variable C - dependent variable D - moderating variable between B and C The framework...

22 October 2020 5,324 3 View

How to quantitative analize the data of TNBS assay for gelatin?

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02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

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28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

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23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

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21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

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21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

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21 February 2021 6,260 3 View

Are exogenous molecules transferred between human cells during doubling ?

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21 February 2021 6,633 3 View

Mohammad Homayoonzadeh Popular answer

Dear Eyu,

I think attached papers are useful for solving your problem.

Sincerely yours, Homayoon.

Christian Q. Scheckhuber

I highly recommend 70 % DMF or EtOH. The reason is that the NBT does not fully dissolve in water.

Regards, Christian

Proteek Dasgupta

Hello Eyu Chan Hong

PFA the docs for your query.

Cheers!!

Sadiq S Mareai

i prefer professor Christian Q. Scheckhuber suggestions

Mohammad Homayoonzadeh

Sobiitraa Devii

Dear Mr Proteek Dasgupta ,

Can the calculation example you provided for NBT be applied to calculate for other solutions' preparations?

Regards, Sobiitraa

Hey @Sobiitraa Devii

You can calculate other solutions with the same calculation. You just need to be careful of the solvents and solutes you're using and the total desired volume of solution you need. Also the molecular weight needs to be checked on the bottle label.

Sorry for the late reply