How to prepare OH- terminated Si Wafer?

Hi, Folks. I am working on the ALD project to deposit Al2O3 on the Si wafer from TMA and Water. I have struggled with the preparation of OH- terminated Si wafer. I have both Si(1 0 0) and...

08 February 2021 8,792 2 View

BrdU staining issue?

Hi everyone. I bumped into the difficulty that I couldn't get any signals from my brain section in the DG region of hippocampus. However, I have standardized the BrdU protocol in my brain...

13 January 2021 9,449 0 View

Real Time PCR: What is the justification for acceptable variance within 3 Ct? Is there any publication on this variance?

Hi all, I was not able to find any source with evidential justification for why the acceptable variance for internal control should be within 3 Ct and not other numbers of cycle of amplification....

17 December 2020 9,481 1 View

Methods for multivariate discrete-time time series clustering?

Can somebody suggest a popular method for clustering multivariate discrete-time time-series data ? My dataset is about water quality parameters taken in 6 stations by seasons in 5 years . Thank...

09 December 2020 6,933 12 View

Effective number of species and Wilcoxon Signed Rank Test for diversity between 2 ecosystems: is this accurate?

Hi! So I'm currently studying the diversity difference between 2 ecosystems. I ran a Shannon-Wiener test on site 1 and site 2 and found the indices of 1.525 and 2.165 , respectively. I have done...

22 November 2020 6,798 1 View

How to reproduce the sub-grade of pavements without using soil?

Dear colleagues, In order to evaluate the performance of concrete slabs-on-grade, the sub-grade should also be considered in the testing setup. Using soil or sand to reproduce the sub-grade is a...

20 November 2020 7,921 1 View

How to determination of available silicon in fertilizer by UV-Vis?

Hello everyone! I have a problem with when the determination of available silicon in fertilizer by UV-Vis. I do that following method A 5-Day Method for Determination of Soluble Silicon...

10 November 2020 1,370 10 View

What is the problem with my 10um Nissl stained cell?

Please see the attachment. In my case, I tried a lot of time on the Nissl staining on 10um cryosection. I am not sure which step goes wrong? I make sure my anti-rolling glass is intact. seem like...

07 November 2020 9,568 3 View

Where and how do I specify frequency in the route section of frequency-dependent calculation (Gaussian using Linux)?

Hello everyone. I would like to compute the dynamic hyperpolarizability value of a molecule using Gaussian on Linux OS. I have read that the essential keywords to include for such request are the...

07 November 2020 5,620 4 View

What type of regression should I be using?

I am planning to conduct a study with the following variables: A - independent variable B - intervening variable C - dependent variable D - moderating variable between B and C The framework...

22 October 2020 5,324 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

No title

22 February 2021 9,932 11 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View