Does anyone know how to propagate Tillandsia in vitro?

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How to solve this error while I simulate gidl using tcad ?

There is an error message "interface silicon/oxide not found in grid xxx.tdr file"

26 June 2024 5,551 0 View

How to eliminate imaginary phonon frequency in phonon dispersion of Coved Graphene Nanoribbons?

Dear all, I want to calculate the phonon dispersion of 4CNR-1-0 (structure is in Fig. 2 (b) in this article Designing coved graphene nanoribbons with charge carrier mob... ) and then calculate...

17 June 2024 6,911 2 View

Synthesis poly(N-isopropylacrylamide) through ATRP by using CUBr/PMDETA?

Monomer: NIPAM; Initiator: BiBB; Catalyst: CuBr; Ligand: PMDETA I added CuBr and the mixture was blue, after that, I purged with nitrogen for 30 minutes and the mixture turned green. The mixture...

27 May 2024 9,144 1 View

I cultured microorganisms obtained from the soil on a medium. Can you identify what kind of microorganisms these are?

I cultured microorganisms obtained from the soil on a medium. Can you identify what kind of microorganisms these are? Is it possible that this microorganism is Stenotrophomonas panacihumi?

22 May 2024 875 4 View

How to determine decision limit (CCα) according to CIR 2021/808?

CIR 2021/808 written as "the CCα shall be calculated as follows: Method 1: by the calibration curve procedure according to ISO 11843-1:1997 ( 14) (here referred to as critical value of the net...

13 May 2024 9,481 0 View

Time to vacuum the chamber of the E-beam evaporation system?

We bought the E-beam evaporation system without a loading chamber. until now, we have used this system about 20~30 times for the deposition of Al2O3, Ti, Au, and Al having thicknesses from 50~100...

23 April 2024 5,156 4 View

Testing methods to determine a non-gene-silencing enzyme inhibitor?

I've recently been trying to investigate the inhibition of H2S-synthesising enzymes: CBS, CSE and 3-MST, via PCR. We've ordered EGCG, PAG and i-3MT-3 to inhibit each enzyme respectively, and have...

16 March 2024 392 1 View

What are the statistical approach for servant leadership in F&B?

To have multiple case study exploration among Malaysia restaurants that study the impact of servant leadership, what are the best statistical approaches for the case studies?

01 March 2024 2,737 1 View

How to reduce the collapse of microfluidic chamber?

During the fabrication of the microfluidic chamber (check its structure in the file, a 5x2.4mm microfluidic chamber with height of 20 μm), the ceilings of the chamber often collapse when bonding...

27 February 2024 6,330 3 View

Why it is necessary to dilute the concentration of surfactant during the process of crosslinking of droplet microfluidic produced calcium alginate?

I see in some references like doi: 10.1021/acs.analchem. 1c02335 and /10.1016/j.colsurfb.2022.113114, mention that it is necessary to dilute the concentration of surfactant during the process of...

27 February 2024 9,571 2 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...

21 July 2024 7,284 4 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

Raghad Mouhamad

https://core.ac.uk/download/pdf/82350829.pdf

https://www.omicsonline.org/open-access/cuttings-as-an-alternative-for-propagation-of-tillandsia-recurvata-anepiphytic-species-of-ethnobotanic-value-2376-0354-1000202.pdf

https://pdfs.semanticscholar.org/76ec/300b80fe10283c665f5c8574180947635e3d.pdf

Arvind Singh

Please take a look at this link.