One-way ANOVA requires n=30. However, of two hormones (BAP and 2-iP) tested at different concentrations (0, 1, 2, 3, and 4 mg/L), in order to achieve 30 samples or replicates, we can do many ways as follow:
A) Do ALL concentrations of TWO hormones with 30 samples at one time,
B) Do ALL concentrations of TWO hormones with ONLY 3 samples but repeated 10 times,
C) Do ALL concentrations of TWO hormones with ONLY 5 samples but repeated 6 times,
D) Do ALL concentrations of TWO hormones with ONLY 10 samples but repeated 3 times,
E) Do ONE of the concentrations of ONE hormone first for 30 samples, experiment is repeated by changing the concentrations or the type of hormone used.
May I know which method is the most suitable? Why?
I was being told that, by repeating more times, I can't use one-way ANOVA already even it is fulfilled the assumptions (n=30, normal and equal var among each population)...by increasing the replicates, I have to use more complicated analysis like two-way ANOVA/ multivariate analysis...Any experts can help me for this also?
Dear Eyu
You perform the experiment according to fixed model so, you must chose your question from these options, and the results will be the answer for this specific question.
In the case, that you include more than one question, then you will add anew variable to your study.
Good Luck
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