Hello. In my laboratory we need to perform the quantification of curcumin in some mice organs after subcutaneous administration of that molecule (“biodistribution” bioanalysis). Our experimental groups are treated and untreated mice, in two different ways (schemes) and we will take samples at different times (days) to check the clearance of the molecule. What we have observed a priori is that curcumin is retained at the site of the injection. We want to use a simple method, such as absorption or fluorescence spectroscopy, and not necessarily HPLC because we don’t have that equipment and we also think it’s not that necessary. Please, I need publications about this approach since I did not find any; most of the determinations are by HPLC. Do you have any suggestions about the procedure or the idea? Do you think it’s a correct approach? Methodologically, what we will do first is a liquid-liquid extraction of curcumin using ethyl acetate, then evaporate the solvent and then reconstitute with a suitable organic solvent for measurement (methanol? ethanol?). Additionally, we want to calculate limit of detection, matrix effects (a priori there should not be such effects) and recovery percentage of the sample preparation process. Do you have any information about this? I would appreciate any protocol or method or tips. Thank you very much.
Hi
I dont have any experience but attached article is a basic one and I hope be helpful.
Good luck my friend
Commercially available curcumin, a bright orange-yellow color pigment of turmeric, consists of a mixture of three curcuminoids, namely, curcumin, demethoxycurcumin, and bisdemethoxycurcumin. These were isolated by column chromatography and identified by spectroscopic studies. The purity of the curcuminoids was analyzed by an improved HPLC method. HPLC separation was performed on a C18 column using three solvents, methanol, 2% AcOH, and acetonitrile, with detection at 425 nm. Four different commercially available varieties of turmeric, namely, Salem, Erode, Balasore, and local market samples, were analyzed to detect the percentage of these three curcuminoids. The percentages of curcumin, demethoxycurcumin, and bisdemethoxycurcumin as estimated using their calibration curves were found to be 1.06 ± 0.061 to 5.65 ± 0.040, 0.83 ± 0.047 to 3.36 ± 0.040, and 0.42 ± 0.036 to 2.16 ± 0.06, respectively, in four different samples. The total percentages of curcuminoids are 2.34 ± 0.171 to 9.18 ± 0.232%.
Improved HPLC Method for the Determination of Curcumin, Demethoxycurcumin, and Bisdemethoxycurcumin
Guddadarangavvanahally K. Jayaprakasha, Lingamullu Jagan Mohan Rao, and Kunnumpurath K. Sakariah*Central Food Technological Research Institute, Mysore 570 013, India
J. Agric. Food Chem., 2002, 50 (13), pp 3668–3672
DOI: 10.1021/jf025506a
Publication Date (Web): May 18, 2002