Hello. I want to standardize a platform to prepare micrometric (900-1200 nm) drug nanosuspensions (nanocrystal dispersions), at my laboratory, of curcumin and other hydrophobic drugs. I have read about preparation methods and we are going to select first a bottom-up procedure since we don't have the equipment to employ a top-down approach. So, I know the main steps: dissolve hydrophobic compound in organic solvent and then mix it with an aqueous solution with a stabilizer. In my laboratory I have a bath sonicator, would that be enough to produce a narrow PDI? What's the setting and the procedure of the mixing of the phases and the stirring/sonication? Do you have any specific protocol paper about this? Thanks a lot.
Hi David,
Regarding to your above question;
3 transdermal curcumin formulations and their ingredients are described below:
Curcumin propylene glycol liposome (PGL) preparation. Hydrogenated phosphatidylcholine 10 mg (HPC), cholesterol 5 mg (Chol), Tween-80 1 mg and curcumin 3 mg were added into propylene glycol. Then 7 ml 5% trehalose solution was added slowly into 3 ml propylene glycol solution with constant rotation of 750 r/min. Curcumin PGL was formed in solution after 10 min constant rotation.
Curcumin liposome preparation. Curcumin liposomes were prepared by ethanol injection method with the same component proportion as curcumin PGL. HPC, Chol, Tween 80 and curcumin were dissolved in 3 ml anhydrous ethanol. Then ethanol solution was added slowly into 10 ml distilled water with constant rotation. Ethanol in final liposome solution was removed by vacuum rotation evaporator. Curcumin liposome was formed after the removal of ethanol.
Curcumin ethosome preparation. Curcumin liposomes were prepared by ethanol injection method with the same component proportion as curcumin PGL. HPC, Chol, Tween 80 and curcumin were dissolved in 3 ml anhydrous ethanol. Then ethanol solution was added slowly into 7 ml distilled water with constant rotation with constant rotation of 750r/min. Curcumin ethosome was formed in solution after 10 min constant rotation.
[Zhao YZ, Lu CT, Zhang Y, Xiao J, Zhao YP, Tian JL, Xu YY, Feng ZG, Xu CY. Selection of high efficient transdermal lipid vesicle for curcumin skin delivery. International journal of pharmaceutics. 2013 Sep 15;454(1):302-309.]
Article Select High Efficient Transdermal Lipid Vesicle For Curcumin...
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