35 Questions 46 Answers 0 Followers
Questions related from Chinkyu Lee
Hello, today i want to ask about ripa buffer, buffer used in Western blot.(Very simple but seemingly important question) You may already know this, but i found out that there are numerous...
10 October 2017 6,688 3 View
Hello, I want to ask about the method of analyzing phospho form and total form of proteins by western blot. I learned that many people conduct stripping the membranes to study expression level...
10 October 2017 2,361 2 View
Hello (I will say my genes of interest as A,B and C) Based on the results so far, A seems to induce translocation of B into nucleus from cytoplasm. As for B, B is already known as a...
07 July 2017 7,044 6 View
Hello, Today i want to ask about films used for detection during western blot. When i conduct detection, i know quite well that film should not be in developer solution too long to avoid...
04 April 2017 6,277 3 View
Hello Today i want to ask very simple but seemingly important question. When conducting western blot, i have to see the expression of phospho form of protein(Protein of my interest). To measure...
02 February 2017 2,844 3 View
Read 2 answers by scientists with 1 recommendation from their colleagues to the question asked by Chinkyu Lee on Dec 24, 2016
12 December 2016 1,697 2 View
Hello, I always get great support and help from Researchgate, And i am sorry to anyone whom i forgot to express my gratitude for reply. Some days ago, i asked same question about sample buffer...
11 November 2016 4,530 4 View
Hello, Today I want to ask whether there is absolute way of extracting proteins from cells for western blot. I found out that there are numerous ways to extract proteins. In my case, I treat...
10 October 2016 6,392 7 View
Hello, I am always grateful for those who answer my questions. I learn a lot thanks to such help. I want to ask question about developer and fixer, required for western blot. I asked quite a...
09 September 2016 3,380 2 View
Hello, Today i want to ask about ratio of developer and fixer in western blot. According to instruction, the dilution ratio of developer and fixer is both 1:4(for example, 200ml developer or...
09 September 2016 10,260 3 View
Hello, This time I want to ask two things about detection during western blot. 1) Casette, required for detection during western blot, is closed tightly during detection so I think pressure is...
08 August 2016 8,036 6 View
Hello, I always get great information from researchgate. It seems that i sometimes forget grateful responses to those who replied my questions and I am sorry for that. Today i want to ask optimal...
08 August 2016 7,851 11 View
Hello, Today I want to get advice on my analysis on research itself rather than technical factors on my research. After making specific hypothesis, the reason why the repeated conductions on one...
08 August 2016 8,197 5 View
Hello, Just few days ago, I asked almost same question about unwanted mixing of ECL during western blot and I got great helps from replies. I appreciate those who responded again. But I think that...
07 July 2016 7,496 2 View
Hello, Today I want to ask about ECL required for detection in western blot. I prepare transparent vinyl to cover ecl-treated membrane units before detection during western blot. I hope you will...
07 July 2016 5,521 9 View
Hello,I always appreciate deeply for all the replies from Researchgate People.Today I want to ask about second question about angiogenic behaviors of HUVECs.I learned that outside cell work tools...
07 July 2016 8,622 2 View
Hello I have been getting great helps from people researchgate. Today I want to ask about markers of HUVECs I isolate HUVECs from umbilical cord and use those cells for my research in...
07 July 2016 426 5 View
Hello I want to appreciate those who helped me with informative answers.And I apologize if I forget to respond your answers. I sometimes may forgetto reply. I know how to isolate HUVECs from...
05 May 2016 2,197 2 View
Hello, I always appreciate deeply for all the replies from Researchgate People. Today I want to ask about second question about angiogenic behaviors of HUVECs. I learned that outside cell...
04 April 2016 6,520 0 View
Hello Today I want to get confirmed about my way of cell caring. I usually keep my cell culture tools(culture dishes, well plates, etc) in stack vertically in incubator in both cases below. 1)...
04 April 2016 7,086 2 View
Hello, Today I want to ask several questions about cell works. I am just a junior in the field of my research, so compared to others, I may ask repetitive and too monotonous questions but I want...
04 April 2016 6,964 4 View
Hello, Today I want to ask about handling of cDNA during RT-PCR. Whatever i do, i think those seem to be trival occasionally affect research results quite critically. I always voltex mixture...
04 April 2016 1,452 7 View
Hello, Thanks to RG members who answered my previous question, I could learn that HUVECs do not change to other Cells without special techniques which induce change of cell type during cell...
04 April 2016 7,786 4 View
Hello, Previously, I asked a question about cDNA samples. I am conducting many RNA works so handling cDNA sample is very important task for me, like any other researchers! From total cDNA...
04 April 2016 9,045 9 View
Hello,I am always cautious on prevention of cell cross contamination.What I express as 'Cross contamination' here is not the mix of cellsof totally different types, but same type of cells treated...
04 April 2016 2,471 9 View
Hello,It is always pleasure for me to get useful information in researchgate.Today I want to ask about the cell passages and states during cell works.As you already know quite well, cells in...
04 April 2016 598 0 View
Hello, I asked similar question previously. And I appreciate all of those who answered my question about handling of cDNA. I want to ask 2 questions. 1. Suppose components in mixture are evenly...
04 April 2016 3,450 4 View
Hi, Today I want to ask about stability of cDNA and protein. I often conduct RT-PCR and Western blotting, starting from huvec culture. During RT-PCR / Western blotting, I wonder whether such...
03 March 2016 6,684 3 View
Hello, I am an graduate student from Korea republic. Before asking a question, I remeber those who answered my previous questions and I want to appreciate them. All answers are great help for...
03 March 2016 2,553 4 View
Hello, I want to ask very simple and easy questions today. I often conduct siRNA transfection to huvec with clean pippet tips and clean 1.5ml tubes. As far as I know, uses of clean pippet tips and...
03 March 2016 6,014 4 View
Hello, I appreciate all of those who helped me with great answers! Today I am curious about types of VEGFs and anlysis of angiogenic behavior of HUVECs. When conducting migration and tube...
03 March 2016 5,828 4 View
Hello everyone, I am an researcher who work with huvec. Several days ago, I did siRNA transfection on huvec to see whether my gene of interest really contributes the migration of huvec. (I have...
02 February 2016 1,152 3 View
Hello, I am working with huvec. I use m199 for huvec works. Here is my question : During experiments with m199, when amount of m199 or trypsin is not enough, I have to use new 20% m199 , new 0%...
01 January 2016 4,330 3 View
Hello! I am doing research with huvec and I have conducted huvec wound healing assay four times with 6 well plates. I studied that gelatin coating should be done before working with huvec, but it...
12 December 2015 1,541 6 View
Hello! I am working with huvec. I have heard that huvec is very sensitive cell and difficult to work with. I have to research with huvec, and I am concerned about the factors which can change...
12 December 2015 9,196 0 View
