RNA and Protein concentration and cell death?(Revised)?

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Best recipe for Ripa buffer?

Hello, today i want to ask about ripa buffer, buffer used in Western blot.(Very simple but seemingly important question) You may already know this, but i found out that there are numerous...

09 October 2017 6,711 3 View

phospho form and total form proteins in Western blot?

Hello, I want to ask about the method of analyzing phospho form and total form of proteins by western blot. I learned that many people conduct stripping the membranes to study expression level...

09 October 2017 2,384 2 View

Research on protein whose localization depends on cell density

Hello (I will say my genes of interest as A,B and C) Based on the results so far, A seems to induce translocation of B into nucleus from cytoplasm. As for B, B is already known as a...

06 July 2017 7,080 6 View

Cautions during detection(Western blot)?

Hello, Today i want to ask about films used for detection during western blot. When i conduct detection, i know quite well that film should not be in developer solution too long to avoid...

03 April 2017 6,300 3 View

Phospho form and total form antibody?

Hello Today i want to ask very simple but seemingly important question. When conducting western blot, i have to see the expression of phospho form of protein(Protein of my interest). To measure...

01 February 2017 2,865 3 View

Quetions or comments about results of western blot?

Hello, I always get great support and help from Researchgate, And i am sorry to anyone whom i forgot to express my gratitude for reply. Some days ago, i asked same question about sample buffer...

10 November 2016 4,548 4 View

Method for extracting proteins for western blot?

Hello, Today I want to ask whether there is absolute way of extracting proteins from cells for western blot. I found out that there are numerous ways to extract proteins. In my case, I treat...

09 October 2016 6,412 7 View

Dilution rate of developer and fixer in western blot?

Hello, Today i want to ask about ratio of developer and fixer in western blot. According to instruction, the dilution ratio of developer and fixer is both 1:4(for example, 200ml developer or...

08 September 2016 10,294 3 View

Questions about developer and fixer?

Hello, I am always grateful for those who answer my questions. I learn a lot thanks to such help. I want to ask question about developer and fixer, required for western blot. I asked quite a...

08 September 2016 3,398 2 View

Important or unimportant factors during western blot?

Hello, This time I want to ask two things about detection during western blot. 1) Casette, required for detection during western blot, is closed tightly during detection so I think pressure is...

07 August 2016 8,052 6 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View