Hello,
I appreciate all of those who helped me with great answers!
Today I am curious about types of VEGFs
and anlysis of angiogenic behavior of HUVECs.
When conducting migration and tube formation of HUVECs,
I learned that human VEGF-A is used for such works.
I wonder whether other types of human VEGF(such as VEGF-C, VEGF-D, etc)
must not be used when analyzing migration and tube formation of HUVECs
and there exist human VEGF types totally not related to angiogenic behaviors of HUVECs above.
As far as I know, the reason why human VEGF-A is recommended is that it is the most efficient type of VEGF to see angiogenic behaviors of HUVECs not because
other kinds of human VEGFs must not be used in the same experiments.
And lastly, VEGF types must be labeled only with big alphabet?(ex) human VEGF-A, human VEGF-B, mouse VEGF-A, etc)
Thank you for reading my a bit long question.
You are correct about labeling. You are also correct that different VEGF family members have different functions and that actually tweak different receptors, e.g., VEGF-A and PlGF or VEGF-B. Some of them work on both angiogenesis and lymphangiogenesis, some only in the latter (like VEGF-D). There is also a dependence on tissue, like VEGF-B is not angiogenic in the cornea. VEGFs are generally weak endothelial mitogens requiring serum for their effects. VEGF-A has been a standard in HUVEC experiments, especially, in the tube formation assay. You might like to use different doses to see the best effect.
Dear Alexander V Ljubimov.
Thank you for your information.
I learned a lot thanks to your help.
But as far as i know, VEGF-C and VEGF-D bind vegf receptor2, which
contributes to angiogenesis(Not lymphangiogenesis.)
If you don't mind, can i ask you about angiogenic effect(Huvec migration in vitro)
of VEGF-C and VEGF-D?
Thank you again?
Dear colleagues.
Six members of the VEGF family, VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E and placental growth factor (PlGF) have been identified to date.
In HUVECs. there are several induced growth factors.
Inhibitor of matrix metalloproteinases depends concentration inhibits tube formation and migration of the human umbilical vein.
- VEGF-A endothelial cells (HUVECs) induced vascular endothelial growth factor A
- VEGF-embryonic angiogenesis (tissue infarction, in particular)
Angiogenesis VEGF-C lymphatics (Lymphangiogenesis)
- VEGF-D is Needed for the development of the lymphatic vascularization of the lungs
PIGF is important for vasculogenesis, angiogenesis during ischemia, inflammation, wound healing and cancer.
Activity of VEGF-A, as the name suggests, has been studied mainly in vascular endothelial cells, although it has an effect on a number of other cell types (for example, stimulates the migration of monocytes / macrophages, acts on neurons, cell cancers, kidney epithelial cells). In vitro it was shown that VEGF-A stimulates endothelial cell mitogenesis and migration. VEGF-A also enhances and increases microvascular permeability and was originally named "vascular permeability factor".
If I understand you correctly, VEGF-C and VEGF-D are not the first inductors of angiogenesis.
If I correctly understand your question, please formulate the question differently, as do examine this question in conjunction with the CD47 antibodies.
I really interest the opinions on this topic.
Thank you
Dear A.V.Petrov,
Thank you very much for your answer and interest.
Yes, I wondered whether Other types(other than human VEGF-A) of human VEGF including human VEGF-C,
human VEGF-D can also be used to analyze angiogenesis of HUVECs but
not efficient as human VEGF-A.
Can I ask how do you think of it?
Sorry, but I am not familiar with CD47.
I will share any information after studying about CD47.
Thank you again.
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