Hello
I have been getting great helps from people researchgate.
Today I want to ask about markers of HUVECs
I isolate HUVECs from umbilical cord and use those cells
for my research in angiogenesis.
I analyze expression of Vegfr1 and Vegfr3 to check what I am working with
are really HUVECs, considering that HUVECs belong to endothelial cells.
(I learn that vegfr1 and vegfr3 are specific markers for endothelial cells.)
But I learn that major marker of HUVECs is vegfr2 rather than vegfr1 and vegfr3. Therefore, i wonder whether analyzing expression of vegfr1 and vegfr3
is rational method to determine that cells I am working with are HUVECs.
In the past, I thought that any markers found to be endothelial markers can be
used for my confirmation described above, but lately i learned that different types of endothelial cells differ in gene expression pattern.
I want to know others' opinion on my work above.
Thank you and hope for great achivements in your research!
Hi Lee,
the Santosh Kumar Guru’s answer seems very comprehensive, although he suggests a large amount of antibodies. In addition to the tube formation assay, I look for a typical marker of HUVE cells such as the von Willebrand factor.
Good luck
We use CD31 and Von Willebrand factor for stainings HUVECs and other endothelial cells
CD144 (Ve-Cadherin) is also specific for EC and there are good antibodies for FACS and staining
We routinely used vWF and PECAM-1(CD31) to check the purity of HUVEC and ECs isolated from pulmonary artery
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