cDNA more than expected?

01 January 2009 0 9K Report

Hi,

I have been using the SuperScript kit to reverse transcribe some polyA tailed mRNA... the strange thing is after purification with RNA hydrolization and precipitation, the amount of cDNA measured with Nanodrop is 7 times the amount of input RNA... I would think that something (unpurified dNTP's, pieces of RNA whatever) is absorbing at the same range... but 7 times??

Does anyone have an explanation for this?

Thanks!

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