21 Questions 6 Answers 0 Followers
Questions related from Bahare Khoshkholgh
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
03 August 2024 3,919 1 View
Hi, I should prapare a standard curve of IAA and I don't know the method is. For measuring of IAA in bacteria, I culture bacteria in TSB medium and mix the supernatant of inoculated medium with...
30 June 2024 4,200 1 View
Hi anyone, I have got in to trouble, We have pet plasmids type 28 and 21 and I have found pet plasmids can't replicate in to pseudomonas, can anyone tell that why pet plasmids, especially, 28 and...
26 June 2024 7,014 1 View
Hi, does anyone khonw which plasmid can we use in cloning in pseudomonas? I have to overexpress two genes in pseudomonas and I don't know which plasmid can replicate and express in pseudomonas....
25 June 2024 9,101 3 View
Hi, Does anyone know pet28 cloning vecot can express in E.coli? I have to clone two genes in to pseudomonas and I dont know whether pet28 plasmid derived from E.coli can whould be successful. On...
25 June 2024 5,230 2 View
I have extracted the plamid of DH5-alpha of E.coli and have done electrophoresis on 1% agarose. But it's alike DNA. Can anyone know what is the size band of its plasmid?
23 June 2024 1,467 3 View
I have done DNA extraction but I didn't have a sharp band, is that a protein contamination?
19 May 2024 1,177 1 View
Hi, I ahve tried dna extraction and when I load DNA in agarose, I have seen dimer like dimerprimer in PCR, can anyone tell me why I saw dimer in dna extraced?
08 May 2024 9,191 4 View
Presently, I am trying DNA extraction of gram negative bacteria and once, I added the mixture of CTAB / NACl after buffer extraction seperately and it was almost good, but I don't know how much I...
27 April 2024 826 0 View
Hi, I am doing DNA extraction of gram negative bacteria (Pseudomonas) for three month. I want to add lyzozyme in dna extraction and I dont know how much is required. I would be grateful if anyone...
23 April 2024 7,957 1 View
It is well known that SDS and CTAB is a good method for removing protein in DNA extraction, but i am doing extraction of dna of pseudomonas bacteria and i didn't see any good results, although i...
10 April 2024 1,857 2 View
I have problem for removing polysccharides in the dna extraction of bacteria. when I want to collect supernatant after adding extraction buffer, suppernatant isn't sufficient and DNA concentration...
10 December 2023 5,450 4 View
Hello I am searching for a suitable 16srRNA primer for identifying bacteria in the genus of pseudomonas. I have found many sequences and I dont know which is the best. Can anyone help me what...
06 June 2023 5,907 4 View
I want to know the stationary phase PGPR pseudomonas such as fluorescence pseudomonas. can anyone help me what is the OD of those bacteria in stationary phase?
05 May 2023 9,514 3 View
I want to add tryptophane to media culture for measuring auxine production in bacteria. I am using chlorometric assay using sallowski sollution. Is tryptophane autoclavable or its better to use...
30 April 2023 8,337 1 View
Hi, Recently, I have done PCR with 16S primer for identifying my bacteria. I have run my control negative with my primers and without DNA. Unfortunately, I saw a band the same size as my pcr...
02 March 2023 6,466 5 View
Hello I have tested the fermentation of carbohydrate by TSI media for bacteria. But the slant of media in the tube became acidic and its color changed to yellow. I don't know what is the result?...
02 December 2022 7,715 0 View
I want to preserve my bacteria in -80 degree. Whould anyone help me say that What is the best medium to preserve bacteria in the long term? I know lots of medium for saving those, but I want to...
08 October 2022 5,812 3 View
Recently, I bought a King B media culture for detection of flueorscence Pseudomonads, but it's made of acid casein, gelatine peptine, potassium phosphate and Mgso4, instead of proteose peptone and...
09 September 2022 8,401 0 View
Is there any way to return the fluerscence properties of the Pseudomonas bacterial strains? Because this fluerscence properties are quickly distroyed under -80 degree, when they have stocked...
05 September 2022 7,686 4 View
I stored my bacteria in -80 degrees, but bacteria have got weak and didn't grow. why did it happen like that? Is it possible that bacteria have destroyed? what is the best method for the storage...
22 June 2022 3,268 1 View
