I want to inoculation of transplant of pepermint with pseudomonas, which I have isolated from the rhizosphere of tomato, but I don't know wich OD I should inoculate with.
According to some liturates, they inoculate of seeds or roots with 10 to the power of 8 of pseudomonas.
In the regards of my bacteria is new, should I gave growth rate of bacteria? two species of them is chlororaphis, the one is glycinsis in the putida group and the other is delftia in betaproteobacteria
An OD600 of 0.6 to 1.0 ( 2-day-old bacterial cells in nutrient broth medium) is commonly advised, as it generally indicates the logarithmic growth phase of bacteria, ensuring active division and viability appropriate for inoculation.
Here, I will introduce a few cited and good papers; make sure to study their materials and methods :
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Article Plant growth‐promoting endophytic bacteria augment growth an...
Article Effects of Phytohormone-Producing Rhizobacteria on Casparian...
Article Bacillus subtilis Inoculation Improves Nutrient Uptake and P...
General method for most bacteria:
You need a separate abundance and wavelength calibration for each bacterial strain.
From the liquid culture medium of each bacterial strain, choose the best wavelength according to the highest absorbance.
Note: The color of the culture medium may be different in all three strains of bacteria. Otherwise, you can use a fixed wavelength of 600 nm.
Subsequently, to establish the correlation between bacterial abundance and absorbance at the selected wavelength, direct measurements of abundance should be conducted using a hemocytometer.
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