What is the best 16srRNA primer for identifying pseudomonas bacteria?

More Bahare Khoshkholgh's questions See All

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,986 1 View

What is the method of standard curve of IAA preparation?

Hi, I should prapare a standard curve of IAA and I don't know the method is. For measuring of IAA in bacteria, I culture bacteria in TSB medium and mix the supernatant of inoculated medium with...

29 June 2024 4,421 1 View

Why pet plasmids can't replicate in pseudomonas?

Hi anyone, I have got in to trouble, We have pet plasmids type 28 and 21 and I have found pet plasmids can't replicate in to pseudomonas, can anyone tell that why pet plasmids, especially, 28 and...

25 June 2024 7,082 1 View

What plasmid is good for cloning in pseudomonas?

Hi, does anyone khonw which plasmid can we use in cloning in pseudomonas? I have to overexpress two genes in pseudomonas and I don't know which plasmid can replicate and express in pseudomonas....

24 June 2024 9,240 3 View

Baseic questions in pseudomonas cloning?

Hi, Does anyone know pet28 cloning vecot can express in E.coli? I have to clone two genes in to pseudomonas and I dont know whether pet28 plasmid derived from E.coli can whould be successful. On...

24 June 2024 5,307 2 View

What is the size band of plasmid of DH5-alpha E.coli?

I have extracted the plamid of DH5-alpha of E.coli and have done electrophoresis on 1% agarose. But it's alike DNA. Can anyone know what is the size band of its plasmid?

22 June 2024 1,589 3 View

How purity is my dna?

I have done DNA extraction but I didn't have a sharp band, is that a protein contamination?

18 May 2024 1,280 1 View

Why I get dimer in dna extraction?

Hi, I ahve tried dna extraction and when I load DNA in agarose, I have seen dimer like dimerprimer in PCR, can anyone tell me why I saw dimer in dna extraced?

07 May 2024 9,269 4 View

How much CTAB/ NACl should I add in the DNA extraction?

Presently, I am trying DNA extraction of gram negative bacteria and once, I added the mixture of CTAB / NACl after buffer extraction seperately and it was almost good, but I don't know how much I...

26 April 2024 893 0 View

How much lyzozyme reauired for dna extraction?

Hi, I am doing DNA extraction of gram negative bacteria (Pseudomonas) for three month. I want to add lyzozyme in dna extraction and I dont know how much is required. I would be grateful if anyone...

22 April 2024 8,035 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,667 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,113 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,056 3 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,986 1 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,672 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,404 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 840 2 View

How to interpret results of AST for Pseudomonas aeruginosa?

While doing AST for Pseudomonas aeruginosa, after incubation, no zone of inhibition observed in the plate near the well. wells surrounded by bacterial growth, when the same plate observed under UV...

25 July 2024 9,229 1 View

Do you mix and add all primers together in a singel PCR reaction (4 total primers for 2 mutations) when using Agilent's multi-site mutagenesis kit?

I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...

25 July 2024 6,517 3 View

Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...

23 July 2024 1,328 5 View

Santosh Kumar Jana

8F and 1492R universal Primer best possible for bacterial identification.

Bahare Khoshkholgh

Tahnks Santosh Kumar Jana

Whould u send me an sample article of that primer? or the sequence?

Okay I will send the sequence

Itto Maroui

Hello,

We used the primers: fD1 (5’-AGAGTTTGATCCTGGCTCAG-3’) et rP2 (5’-ACGGCTACCTTGTTACGACTT-3’).

Article 16S Ribosomal DNA Amplification for Phylogenetic Study