Hello
I have tested the fermentation of carbohydrate by TSI media for bacteria. But the slant of media in the tube became acidic and its color changed to yellow. I don't know what is the result? can anyone help me?
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,986 1 View
Hi, I should prapare a standard curve of IAA and I don't know the method is. For measuring of IAA in bacteria, I culture bacteria in TSB medium and mix the supernatant of inoculated medium with...
29 June 2024 4,421 1 View
Hi anyone, I have got in to trouble, We have pet plasmids type 28 and 21 and I have found pet plasmids can't replicate in to pseudomonas, can anyone tell that why pet plasmids, especially, 28 and...
25 June 2024 7,082 1 View
Hi, does anyone khonw which plasmid can we use in cloning in pseudomonas? I have to overexpress two genes in pseudomonas and I don't know which plasmid can replicate and express in pseudomonas....
24 June 2024 9,240 3 View
Hi, Does anyone know pet28 cloning vecot can express in E.coli? I have to clone two genes in to pseudomonas and I dont know whether pet28 plasmid derived from E.coli can whould be successful. On...
24 June 2024 5,307 2 View
I have extracted the plamid of DH5-alpha of E.coli and have done electrophoresis on 1% agarose. But it's alike DNA. Can anyone know what is the size band of its plasmid?
22 June 2024 1,589 3 View
I have done DNA extraction but I didn't have a sharp band, is that a protein contamination?
18 May 2024 1,280 1 View
Hi, I ahve tried dna extraction and when I load DNA in agarose, I have seen dimer like dimerprimer in PCR, can anyone tell me why I saw dimer in dna extraced?
07 May 2024 9,269 4 View
Presently, I am trying DNA extraction of gram negative bacteria and once, I added the mixture of CTAB / NACl after buffer extraction seperately and it was almost good, but I don't know how much I...
26 April 2024 893 0 View
Hi, I am doing DNA extraction of gram negative bacteria (Pseudomonas) for three month. I want to add lyzozyme in dna extraction and I dont know how much is required. I would be grateful if anyone...
22 April 2024 8,035 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,245 1 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,030 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...
28 July 2024 3,559 0 View
I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...
23 July 2024 3,891 0 View
Sulfuric acid has changed color to yellow.
16 July 2024 6,964 0 View
As PhD student specialized as food and industrial microbiology need to further research on bacteriocin and fermented food.
16 July 2024 7,394 1 View