What is the best method for sterilization of tryptophane?

30 April 2023 1 8K Report

I want to add tryptophane to media culture for measuring auxine production in bacteria. I am using chlorometric assay using sallowski sollution. Is tryptophane autoclavable or its better to use filateration?

Adam B Shapiro

Use a sterile 0.2-micron filter. For small volumes, a syringe filter can be used.

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