Hi, I am doing DNA extraction of gram negative bacteria (Pseudomonas) for three month. I want to add lyzozyme in dna extraction and I dont know how much is required. I would be grateful if anyone tell me how much of lyzozyme should I add in dna extraction.
Thanks
Lysozyme is sometimes used in DNA extraction protocols, particularly when dealing with bacterial cells or certain types of tissues. The amount of lysozyme required can vary depending on factors such as the type and quantity of starting material, the specific DNA extraction protocol being used, and the desired yield and purity of the extracted DNA.
Typically, lysozyme is added to a DNA extraction buffer or solution at a concentration ranging from 1 to 10 mg/ml. However, it's important to consult the specific protocol you're following, as the recommended concentration may vary.
For example, in some protocols for bacterial DNA extraction, a concentration of 1 mg/ml of lysozyme might be sufficient. In other cases, particularly when dealing with tougher cell walls or larger quantities of starting material, a higher concentration of lysozyme may be necessary.
It's also worth noting that lysozyme is often used in combination with other enzymes and reagents (such as proteinase K, SDS, or EDTA) to efficiently lyse cells and degrade proteins, releasing the DNA for subsequent purification steps.
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