Can anyone help me to get a protocol for cresyl violet acetate staining for cultured neural cells?

I undergo differentiation of stem cells into neural cells in 6 well plate and i want to do cresyl violet acetate staining for those cultured cells as a step to confirm differentation.

06 July 2018 2,237 8 View

What is the optimal method for preservation of CSF (cerebrospinal fluid sample) for usage in cell culture as a source of growth factor?

I collect multiple CSf samples and i want to preserve them until collection of all samples complete for usage in cell culture as a source of growth factor. How can i transport the sample? How can...

05 June 2018 5,454 3 View

What is the best anticoagulant (& its conc.)to prevent PRP/culture media from gel formation?

When i add PRP on the culture media it converted from liquid state into gel but i need it in the liquid state . how can i dissolve this problem!

02 March 2018 6,059 0 View

What are the different concentration of a new compound can i start on cancer cell line to calculate IC50?

I have a new compound and i want to calculate IC50 but i dont know what are the concentrations i must start with as a bilot study.

11 December 2017 3,793 0 View

What are the different concentration of a new compound can i start on cancer cell line to calculate IC50?

I have a new compound and i want to calculate IC50 but i dont know what are the concentrations i must start with as a bilot study.

11 December 2017 5,915 8 View

A simple Colorimetric method to measure cell viability for adherent cells in 96-well plates using dye /stain?

The usual methods for measuring cell viability in proliferation cytotoxicity assay is MTT / MTS /.......... I ask for another Inexpensive method using only stain for large number of tested samples.

11 December 2017 7,684 4 View

What is the mechanism of stem cell differentiation ?

I want to Know how stem cell differentiation occur for general either in vivo or in vitro and if this mechanism differ from one cell type to another or not.

03 April 2017 2,812 3 View

How to perform MTS assay on suspended cells like Ehrilch cells or lymphocyte?

31 December 2016 7,889 2 View

How can calculate IC50 ?

11 December 2016 1,639 2 View

What are the difference between PRP and PRF and which method is preferable ?

I am confused between PRP and PRF Which is better!

09 October 2016 8,814 1 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Can RNAscope be used to quantify the mRNA expression level of certain genes?

Hello, all, I want to explore the mRNA expression level of certain genes in the brain under physiological and pathological conditions. In previous studies, the researchers usually isolated the...

18 February 2021 1,518 1 View