I am trying to transfect MEG-01 cells using pSecTag2A vector. Does anyone know the best concentration of Zeocin for selection in this cell line? We tried 250 ug/ml but it did not seem to work.
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...
23 July 2024 3,490 6 View
What should be the frameworks or methodologies to examine written academic ELF? I want to explore the linguistic features of written ELF in research articles.
23 July 2024 4,800 1 View
Thanks
22 July 2024 1,143 3 View
Hi all, Do you know how to plot this figure? I found many of them in Nature journals but have not figured out how to make them. Which software/tool do you suggest? Thank you very much.
15 July 2024 8,384 2 View
The question us regarding catalase activity check of bacterial strains. I saw an article in which it was written that we can use 30%hydrogen peroxide for catalase activity but in our laboratories...
12 July 2024 8,794 2 View
Dear all, May I ask a question about indexed by Scopus. 1 month ago, I have published my paper in the Journal name: Challenges in Sustainability in 20/05/2024. However, this Journal has changed...
06 July 2024 6,734 2 View
Dear Colleagues, I am currently formulating oil in glycerin/water nanoemulsionn of Thymol and Eugenol, using cationic surfactant. After phase diagram, I got trouble that the clear and...
03 July 2024 4,200 2 View
How to calculate Module Young from compressive strength and strain curve
29 June 2024 817 3 View
I have been working in Hanoi University of Public Health. I would like to know where and how I can buy AMOS software?
28 June 2024 6,593 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View