Tuan Huu Nguyen

3 Questions 2 Answers 0 Followers

Questions related from Tuan Huu Nguyen

Zeocin selection for MEG-01 cells?

I am trying to transfect MEG-01 cells using pSecTag2A vector. Does anyone know the best concentration of Zeocin for selection in this cell line? We tried 250 ug/ml but it did not seem to work.

19 January 2024 3,148 0 View

How to prevent activated platelets' exosomes aggregation?

I have been trying to produce exosomes from platelets activated with thrombin. I usually get high yields after ultracentrifugation. However, the exosome pellets are very hard to resuspend and...

25 January 2023 4,702 2 View

Bleeding due to unknown cause?

I am currently working as an attending clinical hematologist. Current I am having to deal with a difficult case of bleeding due to unknown coagulopathy. A 16-year-old man was admitted to our...

01 September 2020 2,598 4 View