Are the random primers only for first-strand cDNA synthesis or can you do one PCR too?

I already did my RT, but now I want to do a PCR to replicate all my cDNA, is it possible to do a PCR with the hexamer random Primer as well? I do not find any KIT for PCR that is not with gen...

13 June 2018 6,254 2 View

You can remove the SDS of gel electrophoresis denaturing?

11 April 2016 7,847 3 View

How could the selection intensity among loci be measured?

I've been genotyping genes that have been under duplication, so I want to know if there is a method to measure selection among those loci under duplication to find differences among them.

15 January 2014 1,632 4 View

Homology modelling or in silico mutation.

I want to get the structure of different proteins. But their similarity is about 95%. There are some mutations distributed in a region of the protein. Which is the best way to obtain a good model,...

11 July 2013 4,219 8 View

Is there a good method/protocol published/available for in vitro siRNA-mediated gene silencing in freshly isolated human monocytes?

Multiple protocols are available to inactivate genes in both macrophages and dendritic cells using electroporation or lipid-based transfection of siRNA pools (not to mention viral...

12 June 2013 3,724 16 View

How to keep good Ethics as Scientific researcher when your research results are linked to market needs and not by search of truth and social needs

For many of us is clear that if as Scientist, we do research for War Industry of any kind, for example, biological or chemical research to produce arms to be used against people, we are in trouble...

03 April 2013 6,834 6 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View