Nisha Rajendran

4 Questions 26 Answers 0 Followers

Questions related from Nisha Rajendran

HnE Protocol for the frozen section of cells encapsulated in hydrogels?

Hi all, I'm facing problems in staining the cryosection of the cells encapsulated in hydrogels. I'm using both charged slides as well as the albumin coated slides for the HnE staining. During the...

05 May 2019 9,704 6 View

What is the procedure for performing ELISA on protein pellet extracted from cell lysate?

Hi all, I wanted to perform ELISA (sandwich elisa) on the protein pellet extracted from cell lysate. I would like to know in which buffer, the protein pellet should be resuspended? Thank you Nisha

12 December 2017 6,319 4 View

Culturing of cells in hydrogel?

Basically i want to trap the cells in the hydrogel. But i'm facing certain problems: 1. Gels are getting detached from the well plate 2. Some of them are dissolving when i add the media to the...

01 January 2017 6,926 7 View

Hi Can anyone explain the difference between papain and pepsin digestion of tissues?

For example, papain is used for retinal digestion and pepsin is used for cornea digestion. How it si different from each other and why??? Thanks Nisha

11 November 2016 4,245 0 View