We ideally need to start from quite some gDNA (>20ug total, >300ng/uL).
Most kits, looked at so far, deliver at most 5ug from 1.5ml culture and we found from 4 prep experiments that a second clear band on agarose gel (
hoi Stephane, ik heb altijd Zymo Yeastar (D2002) gebruikt om DNA uit Pichia te zuiveren (ook vr seq) met goeie opbrengst. Laborimpex is de verdeler.
Hello Astrid!
Thanks a lot for the info.
Others have advised genomic tip100 from the big-Q but I duly note your preference as alternative ;-)
Hope to meet you soon!
Best,
Stephane
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