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Questions related from Stephane Plaisance
We ideally need to start from quite some gDNA (>20ug total, >300ng/uL). Most kits, looked at so far, deliver at most 5ug from 1.5ml culture and we found from 4 prep experiments that a second...
21 June 2017 7,071 2 View
A colleague biologist defined a PPI network of several dozen proteins. He now wants to statistically test if the network is connected more than generally found in the human interactome. We look...
27 April 2016 1,724 3 View
I have tried so far Sc chromosome plugs from Biorad (170-3605), Lonza (50411), and home made from spheroblasts. The Pippin pulse is done with protocol 5-430k 75V 18-22h or with manual protocols...
20 November 2015 4,756 5 View
Can someone motivate the use of 1M beta mercaptoethanol (7.5% v/v) in HMW plant genomic DNA prep? I understand the need for BME to reduce polyphenols and keep reduction state but the '1M' used in...
20 November 2015 4,930 3 View
