Has anyone has any issues with their synthetic minimal media dropout plates only supporting growth for a week or two after being poured? Our lab is having this problem for both S. cerevisiae and S. pombe amino acid dropout plates (rich media is fine, all liquid media is fine). So far it seems the agar itself is the culprit, wanted to see if anyone else has had this problem and has come to any conclusions about the cause.
We had a similar problem and it turned out to be the YNB. Could you share what brand you are using? we fixed by simply changing the brand.
Thanks for your response. We use Difco YNB w/o amino acids + ammonium sulfate for our S. cerevisiae plates, but not for our S. pombe plates.
I am not sure about the pombe,however, we had the problem with the sams brand of ynb it could be a lot specific issue. Just out of curiousness do you mind sharing the lot number of the Ynb?
Hello thank you for the reply. This does not match the lot number we had a problem with. I suggest trying the YNB from formedium. If you are using the same agar for the rich medium (where you mention that you did not have any problem) it would indicate that the agar is not the culprit. Please let me know how it turns out for you.
We think it may be a reaction occurring between the agar and some component in the media- side by side tests pinning onto plates made with old and new agar show that over time, the old agar stops supporting growth, and a new brand/batch of agar seems fine. Very strange.
That is definitely weird but at least it is good to know that it is fixed.
Please check my question at research gate https://www.researchgate.net/post/Do_you_have_any_trouble_with_yeast_dropout_plates_with_Difco_Agar_Cat-214520.
We have had same issue and claimed yeast nitrogen base to BD. And their answer was that their product does not have any problem. Thus we lost our 2kg of yeast nitrogen base without amino acids, because I sent it to them. And we realize that the agar (#2145) inhibit yeast cell growth at dropout plates. I sent the picture listed at research gate question and description to BD technical team for complain. However their answer was "BD does not have established release requirements or performance data for yeast strain BY4741 (ATCC 4040002) on plates produced with Yeast Nitrogen w/o Amino Acids, therefore your complaint will not be confirmed as your testing is being performed out of the claims of our package insert. " Anyway I am going to talk with them more. We lost so much our time, experiments, yeast nitrogen base and agar. They said that they changed #2145 AGAR at 2018. If you still have same issue, please check your agar.
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biochemical Techniques
- Analytical Chemistry
- pH