What are the different post doctoral opportunities available in public health sector?

I am pursuing my PhD in Life sciences. I wanted to continue my career in Public health. Please guide me how to get into the public health sector. Is there any post doctoral opportunities available...

05 June 2018 7,843 3 View

Please suggest me IP grade antibody against Leishmania LPG?

I want to isolate Leishmania donovani LPG interacting host proteins. Kindly share your references for selecting antibody for the same.

31 December 2017 9,082 1 View

Can anybody suggest me protocol for immuno-TEM in human blood derived neutrophils?

08 September 2017 5,850 4 View

Is it advisable to use TIRAP (TLR2 and TLR4 inhibitor) and TAK242 (TLR 4 inhibitor) in case of human neutrophils?

I want to block TLR2 and TLR4 induced signaling in human neutrophils prior to some antigenic stimulation. Since no neutralizing antibody is working in case of neutrophils, so we have decided to...

04 May 2017 6,794 0 View

Can someone suggest a protocol for Phospho-JNK IFA staining in neutrophils?

I am using Phospho-SAPK&JNK pThr183+Tyr185 Antibody (E.665.10) of Thermofisher (Pierce antibody). Protocol followed by me is as follows. 1. PMN fixation with 4% PFA/ 30 min/ 4 degree...

11 December 2015 6,871 4 View

Is there any possible way of maintaining macrophages in a single polarized manner i.e. either M1/M2?

I want to perform infection studies in singularly polarized macrophages i.e. either M1 or M2. How do I maintain THP-1 or primary macrophages in singularly polarized manner?

08 September 2015 6,621 7 View

What is the most reliable and acceptable substitute cell line to human neutrophils?

I need human neutrophils for performing all of my experiments. It is very difficult for me to isolate neutrophils each time and perform experiments. Is their any neutrophil cell line available...

03 April 2015 7,107 13 View

How can I express a protein which contains four rare codons viz. Arg, Leu, Ile and Pro in its sequence?

I am expressing Leishmania protein in bacterial system. This sequence has four rare codons of proline, isoleucine, leucine and arginine. I have tried BL-21 DE3, BL-21 Gold, BL-21 RIL, BL-21 RP,...

01 February 2015 9,622 6 View

Does anybody have generated antibody against Leishmania metacaspase?

I want to generate antibody against Leishmania donovani metacaspase (LdMCA). since I am new to this field of antibody generation. Please guide me how do I approach to achieve my goal. Can anyone...

11 December 2014 1,670 6 View

Is their any IHC method of ROS and NO detection?

I want to detect ROS and NO generation in primary macrophages. DCF-DA and DAF-FM are the methods used by my lab for ROS and NO detection by Fluorimetric analysis. They do this by trypsinizing the...

08 September 2014 3,275 8 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View