Durgesh Pitale

13 Questions 24 Answers 0 Followers

Questions related from Durgesh Pitale

What are the different post doctoral opportunities available in public health sector?

I am pursuing my PhD in Life sciences. I wanted to continue my career in Public health. Please guide me how to get into the public health sector. Is there any post doctoral opportunities available...

06 June 2018 8,050 3 View

Please suggest me IP grade antibody against Leishmania LPG?

I want to isolate Leishmania donovani LPG interacting host proteins. Kindly share your references for selecting antibody for the same.

01 January 2018 9,196 1 View

Can anybody suggest me protocol for immuno-TEM in human blood derived neutrophils?

I want to perform Immuno-TEM staining in human blood-derived neutrophils for my antigen. My protein of interest will suppose to be localized on autophagosomes. Please share protocol for the...

09 September 2017 5,969 4 View

Is it advisable to use TIRAP (TLR2 and TLR4 inhibitor) and TAK242 (TLR 4 inhibitor) in case of human neutrophils?

I want to block TLR2 and TLR4 induced signaling in human neutrophils prior to some antigenic stimulation. Since no neutralizing antibody is working in case of neutrophils, so we have decided to...

05 May 2017 6,905 0 View

Can someone suggest a protocol for Phospho-JNK IFA staining in neutrophils?

I am using Phospho-SAPK&JNK pThr183+Tyr185 Antibody (E.665.10) of Thermofisher (Pierce antibody). Protocol followed by me is as follows. 1. PMN fixation with 4% PFA/ 30 min/ 4 degree...

12 December 2015 6,983 4 View

Is there any possible way of maintaining macrophages in a single polarized manner i.e. either M1/M2?

I want to perform infection studies in singularly polarized macrophages i.e. either M1 or M2. How do I maintain THP-1 or primary macrophages in singularly polarized manner?

09 September 2015 6,737 7 View

What is the most reliable and acceptable substitute cell line to human neutrophils?

I need human neutrophils for performing all of my experiments. It is very difficult for me to isolate neutrophils each time and perform experiments. Is their any neutrophil cell line available...

04 April 2015 7,244 13 View

How can I express a protein which contains four rare codons viz. Arg, Leu, Ile and Pro in its sequence?

I am expressing Leishmania protein in bacterial system. This sequence has four rare codons of proline, isoleucine, leucine and arginine. I have tried BL-21 DE3, BL-21 Gold, BL-21 RIL, BL-21 RP,...

02 February 2015 9,743 6 View

Does anybody have generated antibody against Leishmania metacaspase?

I want to generate antibody against Leishmania donovani metacaspase (LdMCA). since I am new to this field of antibody generation. Please guide me how do I approach to achieve my goal. Can anyone...

12 December 2014 1,790 6 View

Is their any IHC method of ROS and NO detection?

I want to detect ROS and NO generation in primary macrophages. DCF-DA and DAF-FM are the methods used by my lab for ROS and NO detection by Fluorimetric analysis. They do this by trypsinizing the...

09 September 2014 3,393 8 View

Is it possible to get resting neutrophils from blood of a blood bank or do I need to isolate them from fresh blood each time?

I am using human neutrophils as a model for an experiment. Each time I need resting neutrophils, so before asking for blood from a blood bank, I need to know whether can I revive resting...

04 April 2014 4,266 1 View

Would you please suggest some manufacturers of oxazine dye CI basic blue 141?

I want to stain for neutrophil granules. Is their any other stain or dye used to stain specifically neutrophils granules?

02 February 2014 4,586 3 View

Is their any cryopreservation protocol for neutrophils?

I have isolated neutrophils in inactivated state as per experimental need. I am searching for neutrophil cryopreservation protocol. I have used FBS+5%DMSO for cryopreservation. But after revival...

01 January 2014 6,014 2 View