Please suggest me IP grade antibody against Leishmania LPG?

I want to isolate Leishmania donovani LPG interacting host proteins. Kindly share your references for selecting antibody for the same.

31 December 2017 9,082 1 View

Can anybody suggest me protocol for immuno-TEM in human blood derived neutrophils?

08 September 2017 5,850 4 View

Is it advisable to use TIRAP (TLR2 and TLR4 inhibitor) and TAK242 (TLR 4 inhibitor) in case of human neutrophils?

I want to block TLR2 and TLR4 induced signaling in human neutrophils prior to some antigenic stimulation. Since no neutralizing antibody is working in case of neutrophils, so we have decided to...

04 May 2017 6,794 0 View

Can someone suggest a protocol for Phospho-JNK IFA staining in neutrophils?

I am using Phospho-SAPK&JNK pThr183+Tyr185 Antibody (E.665.10) of Thermofisher (Pierce antibody). Protocol followed by me is as follows. 1. PMN fixation with 4% PFA/ 30 min/ 4 degree...

11 December 2015 6,871 4 View

Is there any possible way of maintaining macrophages in a single polarized manner i.e. either M1/M2?

I want to perform infection studies in singularly polarized macrophages i.e. either M1 or M2. How do I maintain THP-1 or primary macrophages in singularly polarized manner?

08 September 2015 6,621 7 View

What is the most reliable and acceptable substitute cell line to human neutrophils?

I need human neutrophils for performing all of my experiments. It is very difficult for me to isolate neutrophils each time and perform experiments. Is their any neutrophil cell line available...

03 April 2015 7,107 13 View

How can I express a protein which contains four rare codons viz. Arg, Leu, Ile and Pro in its sequence?

I am expressing Leishmania protein in bacterial system. This sequence has four rare codons of proline, isoleucine, leucine and arginine. I have tried BL-21 DE3, BL-21 Gold, BL-21 RIL, BL-21 RP,...

01 February 2015 9,622 6 View

Does anybody have generated antibody against Leishmania metacaspase?

I want to generate antibody against Leishmania donovani metacaspase (LdMCA). since I am new to this field of antibody generation. Please guide me how do I approach to achieve my goal. Can anyone...

11 December 2014 1,670 6 View

Is their any IHC method of ROS and NO detection?

I want to detect ROS and NO generation in primary macrophages. DCF-DA and DAF-FM are the methods used by my lab for ROS and NO detection by Fluorimetric analysis. They do this by trypsinizing the...

08 September 2014 3,275 8 View

Is it possible to get resting neutrophils from blood of a blood bank or do I need to isolate them from fresh blood each time?

I am using human neutrophils as a model for an experiment. Each time I need resting neutrophils, so before asking for blood from a blood bank, I need to know whether can I revive resting...

03 April 2014 4,149 1 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 6,831 5 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View