What are the different post doctoral opportunities available in public health sector?

I am pursuing my PhD in Life sciences. I wanted to continue my career in Public health. Please guide me how to get into the public health sector. Is there any post doctoral opportunities available...

05 June 2018 7,843 3 View

Please suggest me IP grade antibody against Leishmania LPG?

I want to isolate Leishmania donovani LPG interacting host proteins. Kindly share your references for selecting antibody for the same.

31 December 2017 9,082 1 View

Can anybody suggest me protocol for immuno-TEM in human blood derived neutrophils?

08 September 2017 5,850 4 View

Can someone suggest a protocol for Phospho-JNK IFA staining in neutrophils?

I am using Phospho-SAPK&JNK pThr183+Tyr185 Antibody (E.665.10) of Thermofisher (Pierce antibody). Protocol followed by me is as follows. 1. PMN fixation with 4% PFA/ 30 min/ 4 degree...

11 December 2015 6,871 4 View

Is there any possible way of maintaining macrophages in a single polarized manner i.e. either M1/M2?

I want to perform infection studies in singularly polarized macrophages i.e. either M1 or M2. How do I maintain THP-1 or primary macrophages in singularly polarized manner?

08 September 2015 6,621 7 View

What is the most reliable and acceptable substitute cell line to human neutrophils?

I need human neutrophils for performing all of my experiments. It is very difficult for me to isolate neutrophils each time and perform experiments. Is their any neutrophil cell line available...

03 April 2015 7,107 13 View

How can I express a protein which contains four rare codons viz. Arg, Leu, Ile and Pro in its sequence?

I am expressing Leishmania protein in bacterial system. This sequence has four rare codons of proline, isoleucine, leucine and arginine. I have tried BL-21 DE3, BL-21 Gold, BL-21 RIL, BL-21 RP,...

01 February 2015 9,622 6 View

Does anybody have generated antibody against Leishmania metacaspase?

I want to generate antibody against Leishmania donovani metacaspase (LdMCA). since I am new to this field of antibody generation. Please guide me how do I approach to achieve my goal. Can anyone...

11 December 2014 1,670 6 View

Is their any IHC method of ROS and NO detection?

I want to detect ROS and NO generation in primary macrophages. DCF-DA and DAF-FM are the methods used by my lab for ROS and NO detection by Fluorimetric analysis. They do this by trypsinizing the...

08 September 2014 3,275 8 View

Is it possible to get resting neutrophils from blood of a blood bank or do I need to isolate them from fresh blood each time?

I am using human neutrophils as a model for an experiment. Each time I need resting neutrophils, so before asking for blood from a blood bank, I need to know whether can I revive resting...

03 April 2014 4,149 1 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

Which way will be better to give OGT inhibitors doses through intravenous or orally for pregnant female rats and concentration suggestion?

I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.

16 February 2021 7,850 2 View