Would like to know the best possible method to get total RNA from whole blood sample?

Hi Vrushali,

I agree with Sabine's advice about the TRI Reagent. But I suggest the TRI Reagent LS for liquid samples (e.g. whole blood). Here you can find the protocol: https://www.mrcgene.com/wp-content/uploads/2014/06/TRI-LSMarch2017.pdf

Pro: - cheaper than any of the column based purification kits

- TRI Reagent LS isolates a whole spectrum of RNA molecules rarely

observed in RNA isolated by other methods

Con: - phenol based solution (toxic compound), need a chemical hood to

use the TRI Reagent

- problematic reagent handling in the sample collection sites because

of chemical hood absence

- very time consuming to use in high throughput mode

You have told that it's a clinical study. I have participated in many clinical biomarker finding studies earlier. We have processed app. 4000 blood samples during 5 years of the study (manual and automatic (QIAcube) processing). Related to my positive experiences, I can tell that we have used PAXgene Blood RNA Tube (https://www.qiagen.com/us/shop/sample-technologies/rna/mirna/paxgene-blood-rna-tube-ivd/#orderinginformation) for pheriperal blood collection and effective RNA stabilization with success.

RNA must be purified using the PAXgene Blood RNA Kit (IVD) for molecular diagnostics applications. PAXgene Blood RNA Tubes (IVD)

(https://www.qiagen.com/us/shop/sample-technologies/rna/total-rna/paxgene-blood-rna-kit-ivd/#productdetails)

can be combined with other PAXgene Blood RNA kits for Research Use Only (RUO) applications.

Pro: - ideal for clinical studies (using standard blood collection practice and

equipment)

- specimen transport and storage at room and refrigerated

temperatures

- standardized sample processing

- you can use it IVD (In Vitro Diagnostic) purposes if you use the

PAXgene Blood RNA (IVD) kit for purification

- you can use QIAGEN QIAcube automatic RNA purification with this kit

Con: - more expensive compare to Trizol

- PAxgene tubes can purchase only in 100 pcs pack

I have heard one of my project leader that the Tempus™ Blood RNA Tube was a really bad choice in an earlier biobank study. The RNA stabilization wasn't good. So they have decided to use the PAXgene system dispate of Tempus system.

If you have any questions I can share you my experiences about the upper systems. Feel free to ask!

Bests,

Tamas

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