I am trying to isolate gDNA from sorted CD4 and CD8 T cells. Human PBMCs will be thawed stained and sorted on a FACS Aria. DNA will be isolated immediately after sorting.
What yield of DNA can I expect if I start with 500k sorted CD4 or CD8 T cells?
Some preliminary experiments have given me less than 1ug of gDNA starting with 500k cells.
I am planning on trying out the NEB Monarch DNA isolation kit, PureLink Genomic DNA Mini Kit and the QIAamp DNA Micro Kit. Any suggestions on which kit is better suited to use with lower cell numbers?
DNA extraction from T lymphocyte yield increased according to the cell numbers,number_dependent manner,so 100 ml buffy coat yields~300 million pan T cells has been widely as fesible approach to deliver DNA.the same idea used to storte thr extract of DNA on rnnning on a FACS Aria the isolation.N.B differnt protocol applied accoding to the source T lymhocyte.Thanks
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