Hi all,
I am interested in knowing if there are antibodies that I can use to detect fibrosis in blood cells using flow cytometry. Can anyone with expertise in immunology and flow cytometry please help?
Thanks in advance :)
Some examples:
1. α-Smooth Muscle Actin (α-SMA): α-SMA is a marker of myofibroblasts, which are involved in fibrosis. It can be detected using antibodies such as anti-α-SMA antibodies conjugated to fluorescent dyes suitable for flow cytometry.
2. Fibroblast-specific Protein 1 (FSP1): FSP1, also known as S100A4, is a marker expressed by fibroblasts during fibrotic processes. Antibodies targeting FSP1 can be used to identify fibroblasts in flow cytometry analysis.
3. Collagen I: Collagen I is a major component of the extracellular matrix in fibrotic tissues. Antibodies against collagen I can be used to assess the deposition of collagen in fibrosis. These antibodies can be labeled with fluorochromes for flow cytometry analysis.
4. Platelet-derived Growth Factor Receptor Beta (PDGFRβ): PDGFRβ is a receptor expressed on fibroblasts and is involved in fibrosis. Flow cytometry-compatible antibodies against PDGFRβ can be used to identify and quantify fibroblasts or fibroblast activation.
5. CD45: CD45 is a common marker used to identify leukocytes, including immune cells. In some cases, alterations in immune cell subsets have been associated with fibrosis. Flow cytometry analysis using CD45 antibodies in combination with other markers can provide insights into immune cell populations and their potential involvement in fibrosis.
Hope it helps:credit AI
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