Working with MAB 263 (GHR)?

11 November 2013 0 4K Report

I want to look at GH receptor on bovine lymphocytes and HepG2 cells. I only get positive cells with permeabilisation. But I'm wondering, because I'm working with anti-GHR-AK clone MAB263 and I get only positive cells when I make the cells permeable with Triton-X 100 0,1% 5 min. Has anyone a good protocol for working with MAB 263.

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Science method

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