Phytic acid limits bioavailability of micronutrients: Is challenge for biofortification?

As many researcher have confirmed that phytic acid content basically present in cereals and legumes crop that responsible for limiting bioavailability of micronutrients (Fe and Zn) in seeds. Lets...

31 January 2021 5,556 8 View

Can a compound have LogP value around 0.05 and LogD value around 4.02?

I understand that there might be huge differences between LogD and LogP for ionizable compounds. Normally, logD should be lower than logP for ionizable compounds. But can LogD value be...

05 January 2021 6,714 0 View

How to remove the bubbles in Collagen gel and maintain the transparency?

I am working on the capillary formation of Lymphatic endothelial cells in a collagen matrix. But I am facing a technical problem to handle Collagen. I have made many times; I am not able to remove...

27 December 2020 5,495 3 View

Biofilm Biofertilizers- New Generation biofertilizers?

Biofilm Biofertilizers-It is being applied to adhere the surface of soil and plant matrix containing consortia of various micro-organism that cut down chemical fertilizer. It is cost effective...

12 October 2020 4,883 13 View

Ethics in research publications?

In present scenario, lots of work were done by researchers and no doubt some of these have potential to change mindset of others that visible through benefiting farmers and other stakeholders....

09 October 2020 4,087 7 View

Can I please get some help with a suitable dissertation topic?

I am a PG student working on my dissertation related to effective document control using BIM. Also looking at alternative topic related to lean construction and BIM Can someone please help find...

14 September 2020 7,153 10 View

How to use commands in ansys workbench?

I am trying to input the material data in ansys mechanical with the help of command i have.This experimental data only consists of stress strain value of concrete cubes.I dont know where this code...

02 August 2020 7,769 5 View

How do I give glucose supplements to rabbits after Alloxan intra-peritoneal dose to prevent hypoglycemic shock?

Its study for alloxan-induced diabetes in the rabbit.

14 July 2020 2,869 1 View

How genetic algorithm can be used to find optimal weights and biases of neural network?

Assume a 3-2-1 (3 neurons in the input layer, 2 neurons in the hidden layer and 1 neuron in the output layer) neural network. How genetic algorithm can be used to find optimal weights and biases...

24 May 2020 1,778 2 View

Is there any evidence why COVID-19 cases started to increase in low-income countries after the cases started to decline in developed nations??

Is there any evidence why COVID-19 cases started to increase in low-income countries after the cases started to decline in developed nations? COVID-19 cases continuously now increasing in...

12 May 2020 4,884 8 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

QPCR troubleshooting - what could cause an increasing ROX signal?

Hi everyone, I'm looking for some help troubleshooting a recent qPCR issue I've encountered. I am using fluorescent Taqman probes and a qPCR master mix that includes ROX as a passive reference....

08 February 2021 419 3 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

Is it strictly recommended to run samples in duplicate during SNP genotyping analysis?

02 February 2021 8,877 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Using the Taqman PCR, how does one know which allele is being determined by VIC / FAM?

I am exploring a number of SNPs, unfortunately although the test are ok, I am perplexed because my preliminary results are nearly all against what is reported in the literature. To confirm my...

29 January 2021 3,905 2 View