Hi,

Do you mean to say that , if phagocytic cells engulf the DNA released by apoptotic cancer cell then those phagocytic cells will also get transformed in to cancer cell?

And if that was your question then the answer is No. the phagocytes will chew up everything and neutralize it. It will never get transformed because of engulfing and DNA or viral entity.

In addition I would like to say that in apoptosis the DNA fragmentation is done by CAD and its unique characteristic is ,it cleaves DNA at every interval of 180-185bp length of DNA at internucleosomal region. Hence you will get the fragments of 180bp and also can se fragments which will be multiple of 180bp.

All the best...

Jaykumar is right. Phagocytosis leads to degradation of engulfed materials. Andrey's point makes sense when considering some cells preferentially undergo necrosis than apoptosis. When you consider transformation, you will find my paper interesting..!! Sphere formation after apoptosis..!!

https://www.researchgate.net/publication/233742776_Blebbishields_the_Emergency_Program_for_Cancer_Stem_Cells_Sphere_Formation_and_Tumorigenesis_after_Apoptosis

Article Blebbishields, the Emergency Program for Cancer Stem Cells: ...

Many thanks to all..

Regarding Jaykumar Kambli : Yes sir if phagocytic cells engulf the DNA released by apoptotic cancer cell or necrosis  then those phagocytic cells will also get transformed in to cancer cell..

please fidn the reference article as proof 

Apoptotic HPV positive cancer cells exhibit transforming properties.

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0036766

Regarding Andrey : if DNA is fragmented into pieces of about one DNA topological domain (50-150 kb),this long fragmentation might carry any functional oncogene ,or driver gene ,that in turn can intercalate with the genome of engulfing cell ..which inturn might transform the engulfing cell to become tumor or pre cancerous  ..Thats why the  nature has choose the apoptotic tumor cell ,to undergo 180bp cleavage ,,This is my view.bcoz to avoid formation of tumor in engulfing cell ,albeit engulfing cell use lysosomal enzyme and DNase II to digest the engulfing genomic  particle or nuclei.The cleavage at 180 bp is essential to avoid accidental arise of tumor in engulfying cell  ..

Regarding: Goodwin:  Blebbishields is new to me. many thanks yaar .. 

I thank you all for helping me to explore this topic .. God bless you all 

Interesting and Informative and new to me (binemic strcuture) ,Many thanks sir..

My appreciation that you are firm with the concept of binemic structure,when the entire world goes behind Uninemic. 

Now i am in chaos ,which one is ostensible Uninemic or Binemic 

  I have gone through few lines  of your work and i came to know that spermatozoon and or haploid cell  has two DNA  helices per chromosome ,Moreover  telomere of one DNA  helices helps in tethering the other helices that in turn forms a  circle ...So finally this binemic model states that haploid cell caries two copy of every gene.

Is my  UNDERSTANDING about Binemic strcutre, right sir ...

One more interesting info  to me ,hallmark of apoptosis -DNA fragmentation also take place in Necrotic cell death  , Necrosis cells too undergo internucleosomal  DNA fragmentation (INDF) independent of Capase DNase ...that is with the aid of  DNase Gamma, a Mg2+/Ca2+dependent endonuclease..

So as per this info the DNA ladder assay cant be used only for apoptosis because necrotic cell too undergo  INDF..

Reference article:DNase gamma  Is the Effector Endonuclease for

Internucleosomal DNA Fragmentation in Necrosis..Journal:  Plos ONE.

Sir,as you have mentioned Necrosis prevails in ripe tumors and drives their growth. I agree ,the release of cellular content like HSP protein ,High mobility group box one and nucleic acid of necrotic cell can induce the inflammatory pathway in neighboring tumor cell and or heterotypic cell (TAM,adipocytes,) via DAMP.(TLR,NOD,RIG-1 pathway)..

You have stated another important line , At the late stage only introduction of certain oligopeptides and monoamines into a blood vessel can help.

You mean to say that ,necrosis can be induced in ripe tumor with oligopeptide and monoamines ...Will it be specific to tumor ,can tumor develop resistance to this oligopeptides and monoamines,

How feasible it can be targeted and stable (life activity) in physiological condition are to be considered when working with the topic ..

Thanks again,for sparing your time in helping me to enhance knowledge and introducing new term - binemic structure.and shift in balance between apoptosis and necrosis..

Happy and nice to hear Sure + Rush... 

Sir, there was error in loading the page ,so i tried to send multiple times ,finally all my message has landed safely one at a time .. ...

Keep sharing such interesting info ..All the best for all your endeavor .

Here is the answer for apoptosis+immune cell fusion http://cancerres.aacrjournals.org/content/early/2017/08/30/0008-5472.CAN-17-0522