Hello, I am culturing cells with high glucose conc, Say 24mM (almost analogous to invivo diabetic condition). Will this interfere with protein production ie., will it cause post-translational modifications in protein products and thereby interfere with probing with antibodies or any other analysis in which they are employed ?
Thanks in advance
High glucose concentration will affect a number of signaling pathways (e.g. AGE-RAGE signaling and AMPK pathway) and definitely affect protein synthesis and post translational modifications
High glucose concentration most likely affects signaling pathways leading to transcriptional and PTMs-mediated regulation of protein function. In this regard, the detection of a particular protein using antibodies may or may not be affected depending on the region of the protein that it is recognized by the antibody and where the PTMs occur.
- Badges
- Science method