Is there any micro-array database available to find?
Please try Gene Expression Omnibus from NCBI.
Is there any Invitro model to study depression and to study the effect of Antidepressants effects using plant extract >
31 December 2019 3,429 1 View
Epithelial to mesenchymal transistion during cancer metastases is known. Can any one suggest me what will be size of mesenchymal cell and at what phase of cell cycle does it migrate to distant...
03 April 2018 7,779 2 View
Hello, I am culturing cells with high glucose conc, Say 24mM (almost analogous to invivo diabetic condition). Will this interfere with protein production ie., will it cause post-translational...
05 June 2015 1,061 2 View
I'm working with a new primer for RT-PCR. Even after running gradient PCR with +10 to -10 deg C from annealing temp, we cannot get any outcome. We blasted the primer and found it to be specific....
03 April 2014 5,934 6 View
Different concepts revolve about DMSO usage. Studies denotes use of 0.1% as non-toxic, contemporarily there exist reports that it is toxic, while using it as vehicle (as dissolving medium) for...
31 December 2013 4,012 1 View
Is that beneficial or detrimental? If so which is more beneficial or more detrimental?
11 December 2013 7,601 11 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View
