Depression Invitro model ?

Is there any Invitro model to study depression and to study the effect of Antidepressants effects using plant extract >

31 December 2019 3,295 1 View

Epithelial to mesenchymal transistion : What is size of Mesenchymal cell and at what phase of cell cycle does it migrate ?

Epithelial to mesenchymal transistion during cancer metastases is known. Can any one suggest me what will be size of mesenchymal cell and at what phase of cell cycle does it migrate to distant...

03 April 2018 7,658 2 View

Will in vitro high glucose concentration affect protein probing ?

05 June 2015 951 2 View

How can we get results from running a gradient PCR?

I'm working with a new primer for RT-PCR. Even after running gradient PCR with +10 to -10 deg C from annealing temp, we cannot get any outcome. We blasted the primer and found it to be specific....

03 April 2014 5,827 6 View

DMSO usage?

Different concepts revolve about DMSO usage. Studies denotes use of 0.1% as non-toxic, contemporarily there exist reports that it is toxic, while using it as vehicle (as dissolving medium) for...

31 December 2013 3,893 1 View

What happens when a promoter of gene is hypomethylated or hypermethylated?

Is that beneficial or detrimental? If so which is more beneficial or more detrimental?

11 December 2013 7,477 11 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View