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I am trying to isolate RNA from Trizol and triton x but results are not coming, so help me out. Thanks
07 August 2014 6,589 5 View
I have taken HLA B27 specific primers from literature and tried to amplify them on DNA of an arthritis patient and there was no amplification. There was no amplification, i want to know that...
03 April 2014 9,379 12 View
Please share if you have.
03 April 2014 2,133 3 View
If yes why , please describe briefly.
03 April 2014 4,349 0 View
I did hemolysis with my peptide at different concentration and it was observed that at high concentration the hemolysis was suppressed as compared to low concentration. I am not getting any...
03 April 2014 7,511 0 View
I am not able to find out the antigen specific for typhoid detection.
03 April 2014 341 1 View
Literature shows that klebsiella is also playing a part in arthritis, so can we identify klebsiella HLA by amplyfying with the primers use to amplify HLA on human PBMC.
03 April 2014 5,631 16 View
Thanks.
02 March 2014 7,717 2 View
For expression studies.
02 March 2014 6,133 5 View
Viability was 57%, but how do I know that the cells shown are viable PBMC cells and as these are stored in PBS can I directly add a lysis buffer to extract DNA from these PBMC stored in PBS?
02 March 2014 5,520 4 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...
02 March 2021 10,003 2 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?
28 February 2021 1,577 1 View
I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.
28 February 2021 6,512 3 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.
28 February 2021 5,929 2 View
based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...
26 February 2021 6,799 3 View
