I'm trying to perform an experiment where keratinocytes and fibroblasts have paracrine signaling between them, but I want to keep the two cell groups separate and prevent migration.

My current idea is to use a transwell system with 3 micron pores, with keratinocytes plated on the bottom of the well and fibroblasts seeded on to the mesh.

Fibroblasts generally run 1-15 microns, but I'm wondering if anyone has experience with this type of assay and can tell me if this will truly keep the cell groups separate.

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