I had a miscommunication with a coworker, and as a result I put unfiltered virus harvested from 293T Phoenix media on to my primary fibroblasts. The transduction worked well, but now I have a couple contaminant Phoenix cells on my culture dishes.
I have tried several days of aggressive washing, since the Phoenix cells are easily detached, but that has been unsuccessful so far at removing all of them. The primary cells I have successfully transduced are very precious, so any other ideas at removing the Phoenix cells are appreciated.
As a last resort, I can flow sort the cells into single wells and grow them out from there. Hoping to avoid it for now as it is incredibly time consuming and also stresses out the cells.
HEK derived cells like Phoenix do not like cooler cultivation temperatures unlike fibroblasts (https://pubmed.ncbi.nlm.nih.gov/7276615/).
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