It is widely known that CRISPR Cas9 off targeting may cause cellular mutagenesis, but if the Cas9 cleaved the wrong sequence (off-target) in the homing-drive system (Homing Endonuclease Gene Drive), will it affect the stability of the payload gene to be passed on to the next generation off spring? Would the payload gene still working (recognise the on-target sequence) in the next gen offspring?
Hi Manwar,
Thank you for your answer and your reading recommendations. I just got the chance to discuss this matter with my supervisor (Nikolai Windbichler, who's also an author in the journal that you recommended -- along with Hammond). We came to a conclusion that off-targeting does not really decrease the chance for the homing-drive to spread in the population as the off-target cleavage means the nuclease still successfully managed to cut the target site (even though with an addition of another cut in another site), but the homing, nevertheless, still happened. So it's been sorted. Thank you once again. Have a nice day.
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