My question is: will 3D culture with matrigel or similar products (extracts from basal membrane) increase my differentiation better than 2D monolayer cultures of epithelial cells? Based on your experience - how do epithelial cells spatially organize in a 3D matrix? My cells grow well in 2D but I get low/partial differentiation so far.
Extracellular matrix proteins can influence the characters of epithelial/endothelial cells. I used matrigel not for growing or differentiation of epithelial cells but for performing capillary tube formation/angiogenesis assay. Gelatin, rat tail collagen, fibronectin are commonly used depending on the cell type. In my experiments coating plates with rat tail collagen works best epithelial/endothelial cell cultures' growth (I use rat collagen to grow mainly human brain endothelium, primary mouse brain endothelial cultures) but I dont see any cell differentiation.
It is kind of hard to imagine a well-differentiated cell like epithelial cell or endothelial cell will differentiate further unless they are blast cells. I may be just ignorant about it but I only saw endothelial cell transform to other cell type in primary cell culture. I would like to be educated about this topic.
these could be helpfuf,
http://www.bdbiosciences.com/documents/webinar_2011_02_3D.pdf
http://www.sciencedirect.com/science/article/pii/S104620230300032X
I am dealing with the same issue, good luck!!!
Cell culture in 3D ECM substrata may dramatically improve differentiation and allow morphogenesis of tissue-like structures. You may find some examples in the following papers (full-text available online) and references therein. Good luck.
https://www.researchgate.net/publication/8537136_Retinoids_induce_lumen_morphogenesis_in_mammary_epithelial_cells
https://www.researchgate.net/publication/15430182_Hepatocyte_growth_factor_stimulates_extensive_development_of_branching_duct-like_structures_by_cloned_mammary_gland_epithelial_cells
https://www.researchgate.net/publication/15504899_Paracrine_induction_of_angiogenesis_in_vitro_by_Swiss_3T3_fibroblasts
https://www.researchgate.net/publication/23567051_cAMP-dependent_chloride_secretion_mediates_tubule_enlargement_and_cyst_formation_by_cultured_mammalian_collecting_duct_cells
Article Retinoids induce lumen morphogenesis in mammary epithelial cells
Article Hepatocyte growth factor stimulates extensive development of...
Article Paracrine induction of angiogenesis in vitro by Swiss 3T3 fibroblasts
Article CAMP-dependent chloride secretion mediates tubule enlargemen...
- Badges
- Science topic