I did PCR to amplify BCR-ABL genes and used three gBlocks as my positive controls. I used MyTaq HS Red Mix from Bioline. My sample generated a band representing internal control but all the gBlocks showed no band. All of the gBlocks contained 10^3 copies DNA. I wonder if anyone have experienced this kind of case because it's rare to happen that gBlocks are not amplified while the samples are just fine and amplified since the DNA amount in gBlocks are high (10^3 copies).
https://www.idtdna.com/pages/docs/synthetic-biology/gblocks-user-guide.pdf
I've experienced problems with gBlocks before. Apparently, IDT only guarantees that a certain percentage of the DNA is your actual sequence. In other words, the sequence you designed and ordered can be as little as 40% of the actual DNA. On the other hand, IDT has a very good policy or reorders/re-synthesis. So what I did was call them, explain that the gBlock was not working as it should (and I had many previous orders where it worked fine) and that I though that maybe something was wrong with that particular gBlock. They sent me another tube free of charge.
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