Juli Elisa Udayani

6 Questions 3 Answers 0 Followers

Questions related from Juli Elisa Udayani

Why were my gBlocks not amplified while my samples were amplified well in the same run?

I did PCR to amplify BCR-ABL genes and used three gBlocks as my positive controls. I used MyTaq HS Red Mix from Bioline. My sample generated a band representing internal control but all the...

05 May 2017 4,208 2 View

What are the differences between C3 spacer & phosphate as oligo-modified blockers?

I am planning to use blocking primer for MEMO-PCR method so I will put a blocker in the 3' end. I wonder if C3 spacer and phosphate have differences in efficiency or others.

03 March 2016 3,958 0 View

Why my melting curve analysis results from same samples change in different PCR run?

I did COLD-PCR for JAK2 wildtype and mutant samples. The previous results were okay, the peaks were around 76-77 celcius. Now the peaks are around 77-78, I wonder what happened. My samples are...

03 March 2016 1,672 5 View

What primer is best to use for analyzing bacterial endophytes in sweet potato tuber?

I want to know the bacterial endophytes in sweet potato tuber using PCR-DGGE method but I'm still curious about the primer I should use. I haven't found other research using this method to analyse...

01 January 2015 9,793 1 View

Does v1-v3 primer of 16S rRNA also detect sweet potato mitochondria?

I'm planning to use v1-v3 primer to analyse bacterial endophytes in sweet potato tuber by PCR-DGGE method and I'm wondering if this primer could also detect other DNA despite my target. I'm...

01 January 2015 8,620 2 View

Is there any open-source software to do analysis of bands in the gel resulting from DGGE?

My goal in my research is to analyse the dynamics of bacterial community structures in water samples from a zero-water discharge system. I might not sequence all the bands so I need to know if...

11 November 2014 1,904 1 View