6 Questions 3 Answers 0 Followers
Questions related from Juli Elisa Udayani
I did PCR to amplify BCR-ABL genes and used three gBlocks as my positive controls. I used MyTaq HS Red Mix from Bioline. My sample generated a band representing internal control but all the...
05 May 2017 4,208 2 View
I am planning to use blocking primer for MEMO-PCR method so I will put a blocker in the 3' end. I wonder if C3 spacer and phosphate have differences in efficiency or others.
03 March 2016 3,957 0 View
I did COLD-PCR for JAK2 wildtype and mutant samples. The previous results were okay, the peaks were around 76-77 celcius. Now the peaks are around 77-78, I wonder what happened. My samples are...
03 March 2016 1,671 5 View
I want to know the bacterial endophytes in sweet potato tuber using PCR-DGGE method but I'm still curious about the primer I should use. I haven't found other research using this method to analyse...
01 January 2015 9,792 1 View
I'm planning to use v1-v3 primer to analyse bacterial endophytes in sweet potato tuber by PCR-DGGE method and I'm wondering if this primer could also detect other DNA despite my target. I'm...
01 January 2015 8,620 2 View
My goal in my research is to analyse the dynamics of bacterial community structures in water samples from a zero-water discharge system. I might not sequence all the bands so I need to know if...
11 November 2014 1,903 1 View